Glial cells of the lamprey nervous system contain keratin-like proteins

The Journal of Comparative Neurology
S E MerrickV M Lee

Abstract

Lamprey axons regenerate following spinal cord transection despite the formation of a glial scar. As we were unable to detect a lamprey homologue of glial fibrillary acidic protein (GFAP), a major constituent of astrocytes, we studied the composition of intermediate filament (IF) proteins of lamprey glia. Monoclonal antibodies (mAbs) were raised to lamprey spinal cord cytoskeletal extracts and these mAbs were characterized by using Western blotting and immunocytochemistry. On two-dimensional (2-D) Western blots, five of the mAbs detected three major IF polypeptides in the molecular weight (MW) range of 45-56 kD. Further studies were conducted to determine the relationship between the lamprey glial-specific antigen and other mammalian IF proteins. Antikeratin 8 antibody recognized two of the three polypeptides. Several of the glial-specific mAbs reacted with human keratins 8 and 18 on Western blots. Keratin-like immunoreactivity was found in all parts of the central and peripheral nervous systems in both larval and adult lampreys. The immunocytochemical staining patterns of glial-specific mAbs were indistinguishable on lamprey spinal cord sections. However, on brain sections, two distinct patterns were observed. A subset of mAbs...Continue Reading

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Citations

Feb 19, 2002·Journal of Neuroscience Methods·Alexander C WrightFelix W Wehrli
Feb 10, 2016·Frontiers in Neuroanatomy·Blanca Fernández-LópezMaría Celina Rodicio
Jul 17, 2003·The Journal of Comparative Neurology·Miguel Meléndez-FerroMaría Celina Rodicio
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Apr 28, 2016·The Journal of Comparative Neurology·Li-Qing JinMichael E Selzer
Dec 25, 2003·The Journal of Comparative Neurology·Ivonne Vidal PizarroMichael E Selzer
Feb 27, 2004·The Journal of Comparative Neurology·Guixin ZhangMichael E Selzer
Feb 9, 2005·The Journal of Comparative Neurology·Li-Qing JinMichael E Selzer
Jan 16, 2008·The Journal of Comparative Neurology·Verona Villar-CerviñoMaría Celina Rodicio
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Dec 1, 2020·Frontiers in Neuroscience·Li-Qing JinMichael E Selzer
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