Glial-derived neurotrophic factor regulates intestinal epithelial barrier function and inflammation and is therapeutic for murine colitis

The Journal of Pathology
Dei Kui ZhangHua Tian Gan

Abstract

Although enteric glial cells (EGCs) have been demonstrated to play a key role in maintaining intestinal epithelial barrier integrity, it is not known how EGCs regulate this integrity. We therefore hypothesized that glial-derived neurotrophic factor (GDNF) produced by EGCs might be involved in this regulation. Here we investigated the role of GDNF in regulating epithelial barrier function in vivo. Recombinant adenoviral vectors encoding GDNF (Ad-GDNF) were administered intracolonically in experimental colitis induced by dextran sulphate sodium (DSS). The disease activity index (DAI) and histological score were measured. Epithelial permeability was assayed using Evans blue dye. The anti-apoptotic potency of GDNF in vivo was evaluated. The expression of tumour necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and myeloperoxidase (MPO) activity were measured by ELISA assay and/or RT-PCR. The expression of ZO-1, Akt, caspase-3, and NF-kappaB p65 was analysed by western blot assay. Our results showed that GDNF resulted in a significant reduction in enhanced permeability, inhibited MPO activity, IL-1beta and TNF-alpha expression, and increased ZO-1 and Akt expression. Moreover, GDNF strongly prevented apoptosis in vivo ...Continue Reading

References

Jan 1, 1994·Scandinavian Journal of Gastroenterology·S LangeE Jennische
Sep 1, 1993·Digestive Diseases and Sciences·S N MurthyD J Sedergran
Apr 1, 1997·Proceedings of the Society for Experimental Biology and Medicine·T Y Ma
Feb 19, 1998·Alimentary Pharmacology & Therapeutics·J B Meddings
Apr 2, 1998·World Journal of Surgery·G Rogler, T Andus
May 30, 1998·Scandinavian Journal of Gastroenterology·Z SunR Andersson
Feb 18, 1999·Cancer Immunology, Immunotherapy : CII·A B LentschM J Edwards
Apr 25, 2000·Annual Review of Medicine·K A Papadakis, S R Targan
Jun 27, 2000·Gastroenterology·B E Sands
Sep 7, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A H GitterM Fromm
Sep 12, 2000·Current Gastroenterology Reports·D Hollander
Nov 30, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·M S InanC Giardina
Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·A CornetR S Liblau
Aug 9, 2002·The New England Journal of Medicine·Daniel K Podolsky
Dec 5, 2002·Glia·Julie CabarrocasRoland S Liblau
Jun 14, 2003·Gastroenterology·Martin SteinkampMax Reinshagen
Jul 26, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Michel NeunlistAnne Jarry
Dec 10, 2003·Oncogene·Thomas F FrankeChizuru Sugimoto
Apr 7, 2004·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M Schemann, M Neunlist
Jan 15, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Hua Tian Gan, J D Z Chen
Feb 21, 2006·International Review of Cytology·Saima AijazKarl Matter
Jun 15, 2006·Annual Review of Cell and Developmental Biology·Kunyoo ShinBen Margolis
Sep 13, 2006·Gut·M C ArrietaJ B Meddings
Apr 10, 2007·The Journal of Surgical Research·Lisa S PoritzWalter A Koltun
Jul 4, 2007·Laboratory Investigation; a Journal of Technical Methods and Pathology·Tor C SavidgeMichel Neunlist
May 16, 2008·Journal of Internal Medicine·M NeunlistT Savidge
May 22, 2008·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·V VillanacciK Geboes

❮ Previous
Next ❯

Citations

Apr 20, 2012·Journal of Clinical Periodontology·Naohide YamamotoAkifumi Akamine
Jan 26, 2011·Glia·Gabrio Bassotti, Vincenzo Villanacci
Jan 19, 2012·Journal of the Neurological Sciences·Inmaculada TassetIsaac Túnez
Apr 3, 2012·Experimental Neurology·Damien P Kuffler
Jul 30, 2016·Mediators of Inflammation·Claudio BernardazziHeitor Siffert P de Souza
Nov 4, 2015·International Journal of Immunopathology and Pharmacology·E CapocciaG Esposito
Mar 9, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Camille PochardMalvyne Rolli-Derkinderen
Sep 22, 2018·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Laetitia KermarrecPhilippe Naveilhan
Nov 1, 2018·Annual Review of Physiology·Sabine SchneiderRobert O Heuckeroth
Nov 21, 2012·Nature Reviews. Gastroenterology & Hepatology·Michel NeunlistMalvyne Rolli-Derkinderen
May 13, 2011·The Journal of Biological Chemistry·Dongmin GuPierre D McCrea
Aug 30, 2014·World Journal of Gastroenterology : WJG·Yan-Bo Yu, Yan-Qing Li
May 7, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Michael MeirNicolas Schlegel
Aug 22, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Michael MeirNicolas Schlegel
Aug 19, 2020·Cell Proliferation·Lin LinYanqing Li
Oct 24, 2017·PloS One·Nicolette W DuijvisAnje A Te Velde
Apr 24, 2016·The Journal of Physiology·Vladimir Grubišić, Brian D Gulbransen
Jun 18, 2019·The Journal of Clinical Investigation·Michael MeirNicolas Schlegel
Apr 8, 2020·Cells·Lucas MorelTatiana Michel
Sep 28, 2018·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Sumei Liu
Nov 9, 2018·Frontiers in Immunology·Ourania S KotsiouSotirios G Zarogiannis
Dec 8, 2020·Frontiers in Oncology·Robert YuanEdgar G Engleman
Jan 6, 2021·Cells·Verena Pawolski, Mirko H H Schmidt
Mar 27, 2021·Cellular and Molecular Life Sciences : CMLS·Amy Marie HollandWerend Boesmans
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Laura López-GómezRaquel Abalo

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis