Glial Fibrillary Acidic Protein as Biomarker Indicates Purity and Property of Auricular Chondrocytes

BioResearch Open Access
Satoru NishizawaKazuto Hoshi

Abstract

Instead of the silicone implants previously used for repair and reconstruction of the auricle and nose lost due to accidents and disease, a new treatment method using tissue-engineered cartilage has been attracting attention. The quality of cultured cells is important in this method because it affects treatment outcomes. However, a marker of chondrocytes, particularly auricular chondrocytes, has not yet been established. The objective of this study was to establish an optimal marker to evaluate the quality of cultured auricular chondrocytes as a cell source of regenerative cartilage tissue. Gene expression levels were comprehensively compared using the microarray method between human undifferentiated and dedifferentiated auricular chondrocytes to investigate a candidate quality control index with an expression level that is high in differentiated cells, but markedly decreases in dedifferentiated cells. We identified glial fibrillary acidic protein (GFAP) as a marker that decreased with serial passages in auricular chondrocytes. GFAP was not detected in articular chondrocytes, costal chondrocytes, or fibroblasts, which need to be distinguished from auricular chondrocytes in cell cultures. GFAP mRNA expression was observed in cul...Continue Reading

References

Jun 1, 1978·Journal of Clinical Pathology·A VollerD E Bidwell
Aug 1, 1993·Experimental Cell Research·H RamdiM Lièvremont
Oct 1, 1996·Genome Research·C A HeidP M Williams
Apr 5, 2001·Journal of Biomedical Materials Research·S C ChangL J Bonassar
Nov 20, 2002·Annals of Plastic Surgery·Yanjun ZengGuangshen Chen
Feb 8, 2006·Molecular Aspects of Medicine·Mikael KubistaNeven Zoric
Dec 8, 2007·Facial Plastic Surgery Clinics of North America·Thomas Romo, James M Pearson
Jan 1, 2009·Tissue Engineering. Part a·Yuko FujiharaKazuto Hoshi
Oct 24, 2009·FEBS Letters·Tobias MaierLuis Serrano
Jul 6, 2010·Biochemical and Biophysical Research Communications·Yuji YamazakiYo-Ichi Nabeshima
Apr 17, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ping WeiQian Shi
Nov 5, 2014·Nature Reviews. Neurology·Matthias Preusser
Apr 22, 2015·Tissue Engineering. Part a·Ernst B HunzikerNahoko Shintani
Oct 11, 2016·Journal of Tissue Engineering and Regenerative Medicine·Mei-Chi ChangJiiang-Huei Jeng
Nov 17, 2016·Journal of Translational Medicine·Christoph BartzJeske J Smink
Jan 8, 2017·Journal of Cellular Physiology·Sanshiro KanazawaKazuto Hoshi
Dec 15, 2018·Journal of Neurotrauma·YouRong Sophie SuSherman C Stein

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Methods Mentioned

BETA
PCR
in vitro transcription
enzyme-linked immunosorbent assay
ELISA
Protein Assay
flow cytometry

Software Mentioned

FlowJo
BellCurve Excel
GeneChip Operating Software GCOS

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