Glial glutamate transporter and glutamine synthetase regulate GABAergic synaptic strength in the spinal dorsal horn.

Journal of Neurochemistry
Enshe JiangHan-Rong Weng

Abstract

Decreased GABAergic synaptic strength ('disinhibition') in the spinal dorsal horn is a crucial mechanism contributing to the development and maintenance of pathological pain. However, mechanisms leading to disinhibition in the spinal dorsal horn remain elusive. We investigated the role of glial glutamate transporters (GLT-1 and GLAST) and glutamine synthetase in maintaining GABAergic synaptic activity in the spinal dorsal horn. Electrically evoked GABAergic inhibitory post-synaptic currents (eIPSCs), spontaneous IPSCs (sIPSCs) and miniature IPSCs were recorded in superficial spinal dorsal horn neurons of spinal slices from young adult rats. We used (2S,3S)-3-[3-[4-(trifluoromethyl)benzoylamino]benzyloxy]aspartate (TFB-TBOA), to block both GLT-1 and GLAST and dihydrokainic acid to block only GLT-1. We found that blockade of both GLAST and GLT-1 and blockade of only GLT-1 in the spinal dorsal horn decreased the amplitude of GABAergic eIPSCs, as well as both the amplitude and frequency of GABAergic sIPSCs or miniature IPSCs. Pharmacological inhibition of glial glutamine synthetase had similar effects on both GABAergic eIPSCs and sIPSCs. We provided evidence demonstrating that the reduction in GABAergic strength induced by the inhi...Continue Reading

References

Jan 1, 1989·Annual Review of Neuroscience·R S Zucker
Mar 15, 1967·Biochimica Et Biophysica Acta·O Z Sellinger
Aug 17, 1999·Nature Neuroscience·V N Murthy, C F Stevens
Mar 29, 2001·Nature Neuroscience·T Schikorski, C F Stevens
May 23, 2001·Progress in Neurobiology·N C Danbolt
May 1, 2002·Anesthesiology·T Philip MalanFrank Porreca
Jun 25, 2005·Neuroscience Letters·Han-Rong WengPatrick M Dougherty
Aug 12, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Joachim ScholzClifford J Woolf
Jun 22, 2006·Journal of Neurochemistry·Lasse K BakHelle S Waagepetersen
Aug 18, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shu-Ling LiangDouglas A Coulter
Aug 24, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kaiwen Kam, Roger Nicoll
Jan 18, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Seena S MathewJohn J Hablitz
Jul 18, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hui XuMin Zhuo
Dec 20, 2008·Nature Reviews. Neuroscience·Erin D Milligan, Linda R Watkins
Feb 10, 2009·Nature Neuroscience·Veeramuthu BalakrishnanLaurence O Trussell
May 12, 2009·Nature Neuroscience·Naila Ben Fredj, Juan Burrone
Apr 27, 2010·Nature Neuroscience·Pavel I OrtinskiDouglas A Coulter
Oct 6, 2010·Neuron·Narinobu JugeYoshinori Moriyama
Oct 16, 2010·Nature Medicine·Ke Ren, Ronald Dubner
Nov 19, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Carolina MuscoliDaniela Salvemini
May 6, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Robert F HuntBret N Smith

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Citations

Feb 5, 2015·Journal of Neuroscience Research·Mari-Carmen MarxBrian Billups
Apr 17, 2016·Neurochemistry International·Marco Flores-MéndezArturo Ortega
May 29, 2016·Neurochemistry International·Walden E Bjørn-Yoshimoto, Suzanne M Underhill
Jul 30, 2016·Neuroscience·Jahangir SajjadSiobhain M O'Mahony
Mar 22, 2018·International Journal of Molecular Sciences·Wuxing DongBrian Billups
Nov 22, 2013·Biochemical Society Transactions·Christopher F RoseVladimir Parpura
Aug 28, 2020·Frontiers in Molecular Neuroscience·Lasse OrmelVidar Gundersen
Mar 28, 2018·Bulletin of Experimental Biology and Medicine·K Yu KalitinA E Matukhno
Feb 23, 2019·Nature Reviews. Neuroscience·Dipan C PatelHarald Sontheimer

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