Jan 1, 1989

Glial hyaluronate-binding protein in Wallerian degeneration of dog spinal cord

Glia
A BignamiD Dahl

Abstract

Wallerian degeneration of spinal cord dorsal columns was produced in three dogs by unilateral extradural dorsal rhizotomy at the lower thoracic level. The spinal cord was studied 1 month, 2 months, and 3 months after surgery. Transverse cryostat sections at the site rhizotomy and at the mid-thoracic level were stained by indirect immunofluorescence with antibodies to the glial fibrillary acidic protein (GFAP) and to the glial hyaluronate-binding protein (GHAP). GHAP immunoreactivity was almost unchanged in the degenerated dorsal column 1 month after rhizotomy. After 2 and 3 months, staining with GHAP antibodies was markedly decreased in the gliosed dorsal column at the site of rhizotomy, but it still persisted at the mid-thoracic level. It is concluded that GHAP persists for long periods of time in dorsal columns undergoing Wallerian degeneration, a finding consistent with its putative role as a nonpermissive substrate preventing nerve regeneration in CNS white matter.

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Mentioned in this Paper

Glial Fibrillary Acidic Protein
Nerve Degeneration
Immunocytochemistry
Neuroglia
CD44 Antigens
Wallerian Degeneration
Spinal Cord
Carrier Proteins
VCAN
Canis familiaris

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