Gliclazide treatment of streptozotocin diabetic rats restores GLUT4 protein content and basal glucose uptake in skeletal muscle

Metabolism: Clinical and Experimental
N PulidoA Rovira

Abstract

This study examined whether the treatment of streptozotocin (STZ)-diabetic rats with gliclazide (5 mg/kg body weight twice daily orally) increases muscle glucose uptake. Rats were treated (group G, n = 10) or untreated (group D, n = 11) for 12 days. Normal rats served as controls (group C, n = 11). At the end of the treatment, both basal and insulin-stimulated glucose uptake by the perfused hindquarters were measured. In gastrocnemious muscles, the protein content of GLUT4 and the insulin binding and tyrosine kinase activity of partially purified solubilized insulin receptors were measured. Group G had a lower mean glycemic value during the treatment period than group D (mean +/- SEM, 17 +/- 0.6 v 19.7 +/- 0.5 mmol/L, P < .05), without differences in serum insulin levels. Basal glucose uptake by the hindquarters was significantly higher in group G versus group D (2.8 +/- 0.3 v 1.3 +/- 0.2 mumol/g/h, P < .05), and was not different versus group C (3.6 +/- 0.2 mumol/g/h). Insulin-stimulated glucose uptake was higher (P < .05) in group C compared with the two groups of diabetic rats. Glucose uptake at 10(-7) mol/L insulin was higher in group G than in group D (9.2 +/- 0.6 v 7.0 +/- 0.6 mumol/g/h, P < .05). Both insulin binding and...Continue Reading

Citations

Feb 19, 2010·Biological Trace Element Research·Sivasankari S ManiKandaswamy Muthusamy
Nov 19, 2013·Journal of Dietary Supplements·Haseena Banu Hedayathullah KhanSubramanian Sorimuthu
Dec 23, 2014·Journal of the Science of Food and Agriculture·Parim Brahma NaiduGanapathy Saravanan
Mar 5, 2017·Molecular and Cellular Biochemistry·Parim BrahmanaiduGanapathy Saravanan
Nov 4, 2009·Archivum Immunologiae Et Therapiae Experimentalis·Anna CzechJan Tatoń
Sep 15, 2010·Plant Foods for Human Nutrition·Ganapathy Saravanan, Ponnusamy Ponmurugan
Aug 5, 2008·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·P Palsamy, S Subramanian
Jun 26, 2010·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·G SaravananT Rajarajan
Jan 11, 2011·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Ganapathy Saravanan, Ponnusamy Ponmurugan

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