Glioblastoma-derived cells in vitro unveil the spectrum of drug resistance capability - comparative study of tumour chemosensitivity in different culture systems

Bioscience Reports
Monika Witusik-PerkowskaPawel P Liberski

Abstract

Resistance to cancer drugs is a complex phenomenon which could be influenced by in vitro conditions. However, tumour-derived cell cultures are routinely used for studies related to mechanisms of drug responsiveness or the search for new therapeutic approaches. The purpose of our work was to identify the potential differences in drug resistance and response to treatment of glioblastoma with the use of three in vitro models: traditional adherent culture, serum-free spheroid culture and novel adherent serum-free culture.The experimental models were evaluated according to 'stemness state' and epithelial-to-mesenchymal transition (EMT) status, invasion capability and their expression pattern of genes related to the phenomenon of tumour drug resistance. Additionally, the response to drug treatments of three different culture models was compared with regard to the type of cell death.Multi-gene expression profiling revealed differences between examined culture types with regard to the expression pattern of the selected genes. Functionally, the examined genes were related to drug resistance and metabolism, DNA damage and repair and cell cycle control, and included potential therapeutic targets.Cytotoxicity analyses confirmed that enviro...Continue Reading

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Citations

Feb 25, 2020·Journal of Oncology·Adrianna RutkowskaPiotr Rieske
Feb 3, 2021·International Journal of Molecular Sciences·Imran KhanMustafa Aziz Hatiboglu

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Methods Mentioned

BETA
PCR
electrophoresis
Profiler
flow cytometry
FACS
fluorescence microscopy

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