PMID: 3746346Jun 1, 1986Paper

Gliogenesis in organotypic tissue culture of the spinal cord of the embryonic mouse. II. Autoradiographic studies

Journal of Neurocytology
D Munoz-Garcia, S K Ludwin

Abstract

Organotypic cultures of the spinal cord of the embryonic mouse were subjected to pulses of tritiated thymidine at various times between explanation and 42 days in vitro (DIV). Autoradiography was performed both on cultures fixed immediately at the end of the pulse and on cultures maintained in radioactive-free medium for various periods after the pulse. Quantitative light autoradiographic studies showed a single peak of glial cell proliferation at 9 DIV equivalent to that demonstrated in vivo. The growth rate of glial cells (related to time in culture) decreased along an exponential decay type curve. All these observations were statistically significant when tested against the corresponding null hypothesis. Ultrastructural autoradiography shows that at early stages of the culture, radial glial cells and immature glial cells divided and eventually gave rise to astrocytes and oligodendrocytes. During the period of maximal cell proliferation, tritiated thymidine was incorporated by differentiated astrocytes and ultrastructurally recognizable immature oligodendrocytes. Oligodendrocytes did not divide beyond the stage of active oligodendrocytes (the cells initiating myelination). They were capable of producing dark oligodendrocytes ...Continue Reading

References

Oct 1, 1976·The Journal of Comparative Neurology·R P SkoffA Stocks
Oct 1, 1976·The Journal of Comparative Neurology·R P SkoffA Stocks
Jan 1, 1969·Zeitschrift für Zellforschung und mikroskopische Anatomie·J E Vaughn
Nov 1, 1972·The Journal of Comparative Neurology·A Privat, C P Leblond
Jan 1, 1971·Acta Neuropathologica·L Manuelidis, E E Manuelidis
Apr 21, 1983·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J G ParnavelasA R Lieberman
Jan 1, 1981·Journal of Neuropathology and Experimental Neurology·A PrivatJ Fulcrand

❮ Previous
Next ❯

Citations

Feb 19, 1990·Biochimica Et Biophysica Acta·R H van der PalM Lopes-Cardozo
May 15, 2002·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Eugen DavidsHartmut Lüddens

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.