May 29, 2020

Glioma-derived extracellular vesicles promote tumor progression by conveying WT1

Taishi TsutsuiRikinari Hanayama


Glioma persists as one of the most aggressive primary tumors of the central nervous system. Glioma cells are known to communicate with tumor-associated macrophages/microglia via various cytokines to establish the tumor microenvironment. However, how extracellular vesicles (EVs), emerging regulators of cell-cell communication networks, function in this process is still elusive. We report here that glioma-derived EVs promote tumor progression by affecting microglial gene expression in an intracranial implantation glioma model mouse. The gene expression of thrombospondin-1 (Thbs1), a negative regulator of angiogenesis, was commonly downregulated in microglia after the addition of EVs isolated from different glioma cell lines, which endogenously expressed Wilms tumor-1 (WT1). Conversely, WT1-deficiency in the glioma-derived EVs significantly attenuated the Thbs1 downregulation and suppressed the tumor progression. WT1 was highly expressed in EVs obtained from the cerebrospinal fluid of human patients with malignant glioma. Our findings establish a novel model of tumor progression via EV-mediated WT1-Thbs1 intercellular regulatory pathway, which may be a future diagnostic or therapeutic target.

  • References30
  • Citations


  • References30
  • Citations


  • This paper may not have been cited yet.

Mentioned in this Paper

Cell Line, Tumor
THBS1 gene
Angiogenic Process
Tumor Cells, Uncertain Whether Benign or Malignant
Intercellular Signaling Alteration
Protein Expression

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.

Related Papers

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Dimitry A Chistiakov, Vladimir P Chekhonin
International Journal of Molecular Sciences
Franz L RicklefsKatrin Lamszus
Critical Reviews in Clinical Laboratory Sciences
Clarissa A WhiteheadS S Stylli
American Journal of Physiology. Cell Physiology
Fumihiko UrabeTakahiro Ochiya
© 2020 Meta ULC. All rights reserved