Glioma-targeted dual functionalized thermosensitive Ferri-liposomes for drug delivery through an in vitro blood-brain barrier

Nanoscale
Di ShiThomas J Webster

Abstract

To date, the delivery of therapeutic agents for malignant brain tumors (such as glioblastoma multiforme (GBM)) remains a significant obstacle due to the existence of the blood-brain barrier (BBB). A multitude of delivery systems (hydrogels, micelles, polymeric nanoparticles, etc.) have been proposed, yet many of them exhibit limited tumor-specific inhibition effects. Herein, a drug-encapsulated dual-functionalized thermosensitive liposomal system (DOX@P1NS/TNC-FeLP) was developed for targeted delivery across the BBB. Specifically, a GBM-specific cell-penetrating peptide (P1NS) and an anti-GBM antibody (TN-C) were conjugated onto the liposome surface for targeted delivery. In addition, superparamagnetic iron oxide nanoparticles (SPIONs) and doxorubicin (DOX) were co-loaded inside the liposomes to achieve thermo-triggered drug release when applying an alternating magnetic field (AMF). Results demonstrated that P1NS/TNC-FeLPs readily transported across an in vitro BBB model and displayed a thermo-responsive and GBM-specific cellular uptake as well as drug release profile. Additionally, results from immunofluorescent (IF) staining and RT-qPCR further demonstrated that DOX@P1NS/TNC-FeLPs specifically entered U-87 human GBM cells and...Continue Reading

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Citations

Jul 17, 2020·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Georgios P SkandalakisConstantinos G Hadjipanayis
Sep 29, 2020·International Journal of Nanomedicine·Ping SunWeilin Chen
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Nasrin ZohrehCristina Busuioc
Dec 29, 2020·Frontiers in Bioengineering and Biotechnology·Henry Ruiz-GarciaDaniel M Trifiletti
Feb 25, 2021·Advanced Nanobiomed Research·Nerea Iturrioz-RodríguezGianni Ciofani
Apr 30, 2021·International Journal of Pharmaceutics·Solmaz Mojarad-JabaliHadi Valizadeh
Jun 4, 2021·Cancer Management and Research·Yihao WangWei Xiang
Jun 7, 2021·International Journal of Pharmaceutics·Marcela Tavares LuizMarlus Chorilli
Jun 20, 2021·Advanced Drug Delivery Reviews·Lingxiao ZhangFangyuan Li

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Methods Mentioned

BETA
electrophoresis
transmission electron microscopy
gel filtration
PCR

Software Mentioned

QuantStudio
Zeiss
QuantStudio 6 Flex Real Time PCR System

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