Glis family proteins are differentially implicated in the cellular reprogramming of human somatic cells

Oncotarget
Seo-Young LeeDong-Youn Hwang

Abstract

The ground-breaking discovery of the reprogramming of somatic cells into pluripotent cells, termed induced pluripotent stem cells (iPSCs), was accomplished by delivering 4 transcription factors, Oct4, Sox2, Klf4, and c-Myc, into fibroblasts. Since then, several efforts have attempted to unveil other factors that are directly implicated in or might enhance reprogramming. Importantly, a number of transcription factors are reported to retain reprogramming activity. A previous study suggested Gli-similar 1 (Glis1) as a factor that enhances the reprogramming of fibroblasts during iPSC generation. However, the implication of other Glis members, including Glis2 and Glis3 (variants 1 and 2), in cellular reprogramming remains unknown. In this study, we investigated the potential involvement of human Glis family proteins, including hGlis1-3, in cellular reprogramming. Our results demonstrate that hGlis1, which is reported to reprogram human fibroblasts, promotes the reprogramming of human adipose-derived stromal cells (hADSCs), indicating that the reprogramming activity of Glis1 is not cell type-specific. Strikingly, hGlis3 promoted the reprogramming of hADSCs as efficiently as hGlis1. On the contrary, hGlis2 showed a strong negative eff...Continue Reading

References

Jan 4, 2001·The International Journal of Biochemistry & Cell Biology·D T DangV W Yang
Jul 10, 2007·Nature Genetics·Massimo AttanasioMathias Treier
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Jan 19, 2016·Trends in Cell Biology·Yan XuMiguel A Esteban

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Citations

Sep 11, 2019·Molecular Biology and Evolution·Yuuri YasuokaYasushi Okazaki
Jan 17, 2019·Thyroid : Official Journal of the American Thyroid Association·Marina N NikiforovaYuri E Nikiforov
Jul 10, 2019·Journal of Oncology·Haitong SunZaixiang Tang

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Methods Mentioned

BETA
immunocytometry
transfections
PCR

Software Mentioned

PluriTest

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