Gln 63 of Rho is deamidated by Escherichia coli cytotoxic necrotizing factor-1

Nature
G SchmidtK Aktories

Abstract

The actin cytoskeleton is regulated by GTP-hydrolysing proteins, the Rho GTPases, which act as molecular switches in diverse signal-transduction processes. Various bacterial toxins can inactivate Rho GTPases by ADP-ribosylation or glucosylation. Previous research has identified Rho proteins as putative targets for Escherichia coli cytotoxic necrotizing factors 1 and 2 (CNF1 and 2). These toxins induce actin assembly and multinucleation in culture cells. Here we show that treatment of RhoA with CNF1 inhibits the intrinsic GTPase activity of RhoA and completely blocks GTPase activity stimulated by the Rho-GTPase-activating protein (rhoGAP). Analysis by mass spectrometry and amino-acid sequencing of proteolytic peptides derived from CNF1-treated RhoA indicate that CNF1 induces deamidation of a glutamine residue at position 63 (Gln 63) to give constitutively active Rho protein.

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Citations

Dec 6, 2006·Naunyn-Schmiedeberg's Archives of Pharmacology·Martin VogelsgesangKlaus Aktories
Nov 13, 2010·Naunyn-Schmiedeberg's Archives of Pharmacology·Alexander E LangKlaus Aktories
Oct 1, 2005·Histochemistry and Cell Biology·Jens WaschkeRoger H Adamson
May 8, 2013·Experimental Cell Research·Emmanuel Lemichez, Klaus Aktories
Oct 28, 1998·Trends in Cell Biology·B HendersonJ Hyams
Jul 4, 2001·Nature Reviews. Molecular Cell Biology·G Schiavo, F G van der Goot
Dec 14, 2011·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Bianca De FilippisCarla Fiorentini
Sep 21, 2004·Nature Reviews. Microbiology·Laurent Boyer, Emmanuel Lemichez
Apr 12, 2005·Nature Reviews. Microbiology·Klaus Aktories, Joseph T Barbieri
Jul 8, 2008·Nature Reviews. Microbiology·Kwang Sik Kim
Feb 24, 2001·Cellular Microbiology·B I KazmierczakJ N Engel
Apr 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Joachim H C OrthKlaus Aktories
Nov 24, 2012·Proceedings of the National Academy of Sciences of the United States of America·Qing YaoFeng Shao
Feb 9, 2012·The Journal of Biological Chemistry·Simone ReipschlägerGudula Schmidt
Jul 24, 2013·The Journal of Cell Biology·Masayuki OnishiJohn R Pringle
Feb 21, 2002·Molecular Biology of the Cell·Qize Wei, Robert S Adelstein
Mar 17, 2006·Molecular Biology of the Cell·Laurent BoyerEmmanuel Lemichez
May 18, 2007·Molecular Biology of the Cell·Alessandro Giamboi MiragliaAlessia Fabbri
Oct 7, 2006·Current Opinion in Gastroenterology·A Fasano
May 12, 2000·Critical Care Medicine·C L Carpenter
Feb 10, 2000·Annals of the New York Academy of Sciences·P Boquet
Apr 11, 2008·The Journal of Pharmacology and Experimental Therapeutics·Y DaiN A Muma
Nov 15, 2006·Infection and Immunity·Lisa E MånssonAgneta Richter-Dahlfors
Jun 23, 2006·Infection and Immunity·Loredana FalzanoCarla Fiorentini

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