Gln5 selectively monodansylated substance P as a sensitive tool for interaction studies with membranes

Biochemical and Biophysical Research Communications
C FerrándizC Abad

Abstract

Substance P (SP) is a neuropeptide endowed with several important biological activities both in the central and peripheral nervous system. Taking advantage of the presence of glutamine residues in SP, the peptide was labelled with the fluorescent probe monodansylcadaverine using the transglutaminase (TGase)-reaction in order to study interactions between SP and model or natural membranes. Although it was verified that both adjacent glutamines of the peptide can act as substrate for TGase in a consecutive reaction, conditions were optimized to selectively label Gln5. This fluorescent SP analogue was found to adopt environment-dependent conformations similar to those of the natural peptide and proved to be functionally active on guinea pig trachea. Fluorescence spectroscopy was used to demonstrate the potential use of dansylated SP in studies involving interactions with membranes.

Citations

Jun 28, 2011·Biochemical and Biophysical Research Communications·Yutaro MoriNoriho Kamiya
Jul 4, 2008·Amino Acids·Angelo Facchiano, Francesco Facchiano
Aug 14, 2009·Organic & Biomolecular Chemistry·Noriho KamiyaHiroyuki Jikuya
Sep 8, 2010·Chemical Communications : Chem Comm·Hiroki AbeNoriho Kamiya
Aug 2, 1996·The Journal of Biological Chemistry·E BallestarL Franco
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Aug 2, 2000·International Journal of Biological Macromolecules·C M GómezC Abad
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cristina Bolzati, Barbara Spolaore

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