Global analysis of asymmetric RNA enrichment in oocytes reveals low conservation between closely related Xenopus species

Molecular Biology of the Cell
Maike ClaussenTomas Pieler

Abstract

RNAs that localize to the vegetal cortex during Xenopus laevis oogenesis have been reported to function in germ layer patterning, axis determination, and development of the primordial germ cells. Here we report on the genome-wide, comparative analysis of differentially localizing RNAs in Xenopus laevis and Xenopus tropicalis oocytes, revealing a surprisingly weak degree of conservation in respect to the identity of animally as well as vegetally enriched transcripts in these closely related species. Heterologous RNA injections and protein binding studies indicate that the different RNA localization patterns in these two species are due to gain/loss of cis-acting localization signals rather than to differences in the RNA-localizing machinery.

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Citations

Jan 18, 2017·Genesis : the Journal of Genetics and Development·Mary Lou King
Jun 10, 2020·Computational and Structural Biotechnology Journal·Ravindra NaraineRadek Sindelka
Apr 11, 2021·Nature Reviews. Molecular Cell Biology·Sulagna DasEvelina Tutucci
Jun 9, 2021·Nature Communications·Karoline HollerJan Philipp Junker

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Datasets Mentioned

BETA
GSE58420
BX772720

Methods Mentioned

BETA
PCR
reverse transcription PCR
GTPase
dissection
in vitro transcription
confocal microscopy
affinity purification

Software Mentioned

FastQC
Illumina BaseCaller
R
Bioconductor
BLAST
biomaRt
DAVID
Samtools
ImageJ
Xenbase

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