Global assessment of Antrodia cinnamomea-induced microRNA alterations in hepatocarcinoma cells

PloS One
Yen-Ju ChenKuo Ping Chiu

Abstract

Recent studies have demonstrated a potent anticancer potential of medicinal fungus Antrodia cinnamomea, especially against hepatocarcinoma. These studies, however, were performed with prolonged treatments, and the early anticancer events remain missing. To probe the early anticancer mechanisms of A. cinnamomea, we treated SK-Hep-1 liver cancer cell with A. cinnamomea fruiting body extract for 2 and 4 hours, sequenced RNA samples with next-generation sequencing approach, and profiled the genome-wide miRNA and mRNA transcriptomes. Results unmistakably associated the early anticancer effect of A. cinnamomea fruiting body extract with a global downregulation of miRNAs which occurred solely in the A. cinnamomea fruiting body extract-treated SK-Hep-1 cells. Moreover, the inhibitory effect of A. cinnamomea fruiting body extract upon cancer miRNAs imposed no discrimination against any particular miRNA species, with oncomirs miR-21, miR-191 and major oncogenic clusters miR-17-92 and miR-106b-25 among the most severely downregulated. Western blotting further indicated a decrease in Drosha and Dicer proteins which play a key role in miRNA biogenesis, together with an increase of XRN2 known to participate in miRNA degradation pathway. Tran...Continue Reading

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Citations

Dec 2, 2015·The American Journal of Chinese Medicine·Ying-Yi ChenMing-Jyh Sheu
Feb 7, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zhe ZhangPeng Ye
Sep 30, 2021·Expert Reviews in Molecular Medicine·Sepideh MirzaeiHaroon Khan

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Datasets Mentioned

BETA
GSE48327

Methods Mentioned

BETA
ELISA
PCR
electrophoresis
RNA-Seq

Clinical Trials Mentioned

NCT01134016

Software Mentioned

Cufflinks
Bowtie
DAVID
Cutadapt
Dicer
Drosha
shell
SOLiD
ggplot2

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