Global changes in gene expression during compatible and incompatible interactions of cowpea (Vigna unguiculata L.) with the root parasitic angiosperm Striga gesnerioides

BMC Genomics
Kan HuangMichael P Timko

Abstract

Cowpea, Vigna unguiculata L. Walp., is one of the most important food and forage legumes in the semi-arid tropics. While most domesticated forms of cowpea are susceptible to the root parasitic weed Striga gesnerioides, several cultivars have been identified that show race-specific resistance. Cowpea cultivar B301 contains the RSG3-301 gene for resistance to S. gesnerioides race SG3, but is susceptible to race SG4z. When challenged by SG3, roots of cultivar B301 develop a strong resistance response characterized by a hypersensitive reaction and cell death at the site of parasite attachment. In contrast, no visible response occurs in B301 roots parasitized by SG4z. Gene expression in the roots of the cowpea cultivar B301 during compatible (susceptible) and incompatible (resistant) interactions with S. gesnerioides races SG4z and SG3, respectively, were investigated at the early (6 days post-inoculation (dpi)) and late (13 dpi) stages of the resistance response using a Nimblegen custom design cowpea microarray. A total of 111 genes were differentially expressed in B301 roots at 6 dpi; this number increased to 2102 genes at 13 dpi. At 13 dpi, a total of 1944 genes were differentially expressed during compatible (susceptible) intera...Continue Reading

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Citations

Oct 16, 2015·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Kai Kristoffer LieMari Moren
Jul 12, 2013·Molecular Plant Pathology·Thomas SpallekKen Shirasu
Sep 1, 2015·Frontiers in Plant Science·Kanako MitsumasuSatoko Yoshida
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Jul 25, 2014·Journal of Experimental Botany·Fabien SénéchalJérôme Pelloux
Oct 20, 2018·Plant Methods·Lidiane Lindinalva Barbosa AmorimAna Maria Benko-Iseppon
Dec 13, 2017·The Plant Journal : for Cell and Molecular Biology·Harihar Jaishree SubrahmaniamFabrice Roux
Nov 18, 2020·The New Phytologist·J Musembi MutukuKen Shirasu
Dec 3, 2020·International Journal of Molecular Sciences·Luyang HuWeijun Zhou
Dec 23, 2020·International Journal of Molecular Sciences·Sandeep Kaur DhaliwalPrashant Kaushik
Apr 2, 2021·Plant Physiology·Muhammad JamilSalim Al-Babili

Methods Mentioned

BETA
PCR
Chip

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