Global control of cysteine metabolism by CymR in Bacillus subtilis

Journal of Bacteriology
Sergine EvenIsabelle Martin-Verstraete

Abstract

YrzC has previously been identified as a repressor controlling ytmI expression via its regulation of YtlI activator synthesis in Bacillus subtilis. We identified YrzC as a master regulator of sulfur metabolism. Gene expression profiles of B. subtilis delta yrzC mutant and wild-type strains grown in minimal medium with sulfate as the sole sulfur source were compared. In the mutant, increased expression was observed for 24 genes previously identified as repressed in the presence of sulfate. Since several genes involved in the pathways leading to cysteine formation were found, we propose to rename YrzC CymR, for "cysteine metabolism repressor." A CymR-dependent binding to the promoter region of the ytlI, ssuB, tcyP, yrrT, yxeK, cysK, or ydbM gene was demonstrated using gel shift experiments. A potential CymR target site, TAAWNCN2ANTWNAN3ATMGGAATTW, was found in the promoter region of these genes. In a DNase footprint experiment, the protected region in the ytlI promoter region contained this consensus sequence. Partial deletion or introduction of point mutations in this sequence confirmed its involvement in ytlI, yrrT, and yxeK regulation. The addition of O-acetylserine in gel shift experiments prevented CymR-dependent binding to ...Continue Reading

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Jan 1, 1987·Methods in Enzymology·K Soda
Nov 1, 1971·Journal of General Microbiology·C KariF Hernádi
Jun 27, 1998·Journal of Bacteriology·M M NakanoA L Sonenshein
Oct 23, 1998·Microbiology·Jan R van der PloegIan F Connerton
Jan 19, 1999·Annual Review of Microbiology·M M Nakano, P Zuber
Nov 26, 1999·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·A Sekowska, A Danchin
Jun 16, 2001·Advances in Microbial Physiology·M Hecker, U Völker
Jul 11, 2001·FEMS Microbiology Letters·J R van der PloegT Leisinger
Sep 5, 2001·Molecular Microbiology·C W PriceP Youngman
Sep 7, 2001·Journal of Bacteriology·A PetersohnM Hecker
Dec 14, 2001·Proceedings of the National Academy of Sciences of the United States of America·C J SchwartzP J Kiley
May 23, 2002·BMC Microbiology·Agnieszka Sekowska, Antoine Danchin
Aug 10, 2002·Journal of Bacteriology·Isabelle GuillouardIsabelle Martin-Verstraete
Aug 24, 2002·Journal of Bacteriology·Sandrine AugerIsabelle Martin-Verstraete
Mar 6, 2003·Journal of Bacteriology·Lars Ingo Ole LeichertMichael Hecker
Mar 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Brooke A Murphy McDanielTina M Henkin
Mar 3, 2004·Research in Microbiology·Marie-Françoise HulloIsabelle Martin-Verstraete
Mar 5, 2004·Journal of Bacteriology·James K LithgowSimon J Foster
Jun 25, 2004·Nucleic Acids Research·Dmitry A RodionovMikhail S Gelfand
Jul 21, 2004·Journal of Bacteriology·Pierre BurguièreIsabelle Martin-Verstraete
Sep 18, 2004·Journal of Bacteriology·Elisabeth HärtigMichiko M Nakano
May 20, 2005·Journal of Bacteriology·Brice SperandioEric Guédon

❮ Previous
Next ❯

Citations

Jan 17, 2013·Critical Reviews in Food Science and Nutrition·María Del Carmen Martínez-CuestaTeresa Requena
Dec 21, 2010·Applied and Environmental Microbiology·Marie-Pierre ForquinIsabelle Martin-Verstraete
Jan 5, 2012·Infection and Immunity·Vahid Farshchi AndisiJetta J E Bijlsma
Aug 4, 2006·Journal of Bacteriology·Michiko M NakanoKazuo Kobayashi
Oct 20, 2009·Journal of Bacteriology·Dierk-Christoph PötherMichael Hecker
Oct 24, 2006·Journal of Bacteriology·Marie-Françoise HulloIsabelle Martin-Verstraete
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Ana Gutiérrez-PreciadoEnrique Merino
Sep 9, 2010·BMC Microbiology·Gaelle AndréIsabelle Martin-Verstraete
Oct 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·Danielle Biscaro PedrolliMatthias Mack
Dec 5, 2015·Frontiers in Microbiology·Chiara FerrarioMarco Ventura
Jun 16, 2011·Proteomics·Rebecca SchroeterThomas Schweder
Aug 16, 2013·Environmental Microbiology·Che-Man ChanAgnieszka Sekowska
Mar 30, 2016·Research in Microbiology·Gaelle AndréIsabelle Martin-Verstraete
Feb 3, 2015·Biochimica Et Biophysica Acta·Joana A SantosSandra Macedo-Ribeiro
Jan 4, 2012·FEMS Microbiology Letters·Jennifer KimDennis G Cvitkovitch
Oct 25, 2007·FEMS Microbiology Letters·Galina Yu Kovaleva, Mikhail S Gelfand
Aug 4, 2018·Antioxidants & Redox Signaling·Emma SevillaMaría F Fillat

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.