Global distribution of epidemic-related Shiga toxin 2 encoding phages among enteroaggregative Escherichia coli.

Scientific Reports
Keiko KimataSunao Iyoda

Abstract

Since the Shiga toxin-producing enteroaggregative Escherichia coli (Stx-EAEC) O104:H4 strain caused a massive outbreak across Europe in 2011, the importance of Stx-EAEC has attracted attention from a public health perspective. Two Stx-EAEC O86 isolates were obtained from patients with severe symptoms in Japan in 1999 and 2015. To characterize the phylogeny and pathogenic potential of these Stx-EAEC O86 isolates, whole-genome sequence analyses were performed by short-and long-read sequencing. Among genetically diverse E. coli O86, the Stx-EAEC O86 isolates were clustered with the EAEC O86:H27 ST3570 subgroup. Strikingly, there were only two loci with single nucleotide polymorphisms (SNPs) between the Stx2a phage of a Japanese O86:H27 isolate and that of the European epidemic-related Stx-EAEC O104:H4 isolate. These results provide evidence of global distribution of epidemic-related Stx2a phages among various lineages of E. coli with few mutations.

Associated Datasets

References

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Methods Mentioned

BETA
PCR

Software Mentioned

BLAST
IQ
Unicycler
BactSNP
GenomeMatcher
MUMmer
Hierarchical Genome Assembly Process ( HGAP )
BLASTn
PHASTER
SMRT Analysis

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