Global expression studies in baker's yeast reveal target genes for the improvement of industrially-relevant traits: the cases of CAF16 and ORC2.

Microbial Cell Factories
Roberto Pérez-TorradoFrancisca Randez-Gil

Abstract

Recent years have seen a huge growth in the market of industrial yeasts with the need for strains affording better performance or to be used in new applications. Stress tolerance of commercial Saccharomyces cerevisiae yeasts is, without doubt, a trait that needs improving. Such trait is, however, complex, and therefore only in-depth knowledge of their biochemical, physiological and genetic principles can help us to define improvement strategies and to identify the key factors for strain selection. We have determined the transcriptional response of commercial baker's yeast cells to both high-sucrose and lean dough by using DNA macroarrays and liquid dough (LD) model system. Cells from compressed yeast blocks display a reciprocal transcription program to that commonly reported for laboratory strains exposed to osmotic stress. This discrepancy likely reflects differences in strain background and/or experimental design. Quite remarkably, we also found that the transcriptional response of starved baker's yeast cells was qualitatively similar in the presence or absence of sucrose in the LD. Nevertheless, there was a set of differentially regulated genes, which might be relevant for cells to adapt to high osmolarity. Consistent with t...Continue Reading

References

Feb 1, 1992·The Journal of Applied Bacteriology·M H ZwieteringK van 't Riet
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Feb 1, 1997·Nature Genetics·A Decottignies, A Goffeau
Dec 31, 1997·Molecular and Cellular Biology·D G Lee, S P Bell
Apr 16, 1998·Applied Microbiology and Biotechnology·A C CodónM Korhola
Jun 4, 1999·Trends in Biotechnology·F Randez-GilJ A Prieto
Dec 5, 2000·Molecular Biology of the Cell·A P GaschP O Brown
Feb 17, 2001·Molecular Biology of the Cell·H C CaustonR A Young
Jan 18, 2002·The Journal of Biological Chemistry·Yukifumi Uesono, Akio Toh-E
Jun 1, 2002·Microbiology and Molecular Biology Reviews : MMBR·Stefan Hohmann
Jul 27, 2002·Journal of Biotechnology·José E Pérez-OrtínTrinidad M Alberola
Oct 16, 2002·The Journal of Biological Chemistry·Takehiko SaharaSatoru Ohgiya
Dec 31, 2003·Advances in Applied Microbiology·An TangheJohan M Thevelein
Aug 10, 2004·The Journal of Biological Chemistry·Laia ViladevallJoaquín Ariño
Mar 5, 2005·Applied and Environmental Microbiology·Joaquin PanaderoJose Antonio Prieto
May 17, 2005·Genome Biology·Karen EilbeckMichael Ashburner
Jun 1, 1990·Applied and Environmental Microbiology·M H ZwieteringK van 't Riet
Aug 2, 2006·FEMS Yeast Research·Lakshmi RamachandranWilliam C Burhans
Aug 29, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Tania M Malavé, Sharon Y R Dent
Feb 15, 2007·FEMS Microbiology Reviews·Jaime AguileraJose Antonio Prieto
Jun 15, 2007·Applied and Environmental Microbiology·Joaquín PanaderoFrancisca Randez-Gil
Sep 29, 2007·Advances in Food and Nutrition Research·Linda F BissonLucy Joseph
Oct 30, 2007·Eukaryotic Cell·Eve LenssenMartine A Collart
Jan 25, 2011·Microbial Biotechnology·Jaime AguileraJose Antonio Prieto

❮ Previous
Next ❯

Citations

Mar 8, 2013·Annual Review of Food Science and Technology·Francisca Randez-GilJosé A Prieto
Sep 24, 2013·Applied and Environmental Microbiology·Elham AslankoohiKevin J Verstrepen
Jan 27, 2016·Journal of Biotechnology·Lidia Ballester-TomásFrancisca Randez-Gil
Apr 15, 2014·FEMS Microbiology Reviews·Jan SteenselsKevin J Verstrepen
Oct 22, 2016·International Journal of Food Microbiology·Ariunzaya TsolmonbaatarHiroshi Takagi
Aug 24, 2017·FEMS Microbiology Reviews·Maria C DzialoKevin J Verstrepen
Sep 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Nore StruyfChristophe M Courtin

❮ Previous
Next ❯

Methods Mentioned

BETA
ESR
PCR
chips
chip

Software Mentioned

ArrayStat
ArrayVision
Statistica
Image Gauge

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.