Global gene expression in Staphylococcus aureus biofilms

Journal of Bacteriology
Karen E BeenkenMark S Smeltzer

Abstract

We previously demonstrated that mutation of the staphylococcal accessory regulator (sarA) in a clinical isolate of Staphylococcus aureus (UAMS-1) results in an impaired capacity to form a biofilm in vitro (K. E. Beenken, J. S. Blevins, and M. S. Smeltzer, Infect. Immun. 71:4206-4211, 2003). In this report, we used a murine model of catheter-based biofilm formation to demonstrate that a UAMS-1 sarA mutant also has a reduced capacity to form a biofilm in vivo. Surprisingly, mutation of the UAMS-1 ica locus had little impact on biofilm formation in vitro or in vivo. In an effort to identify additional loci that might be relevant to biofilm formation and/or the adaptive response required for persistence of S. aureus within a biofilm, we isolated total cellular RNA from UAMS-1 harvested from a biofilm grown in a flow cell and compared the transcriptional profile of this RNA to RNA isolated from both exponential- and stationary-phase planktonic cultures. Comparisons were done using a custom-made Affymetrix GeneChip representing the genomic complement of six strains of S. aureus (COL, N315, Mu50, NCTC 8325, EMRSA-16 [strain 252], and MSSA-476). The results confirm that the sessile lifestyle associated with persistence within a biofilm...Continue Reading

References

Aug 1, 1977·Journal of Bacteriology·B Friedrich, B Magasanik
Mar 1, 1992·Molecular and Biochemical Parasitology·P J SchofieldJ R Wilson
Mar 1, 1991·Infection and Immunity·J V Ligon, G E Kenny
Sep 1, 1986·Microbiological Reviews·R CuninV Stalon
Jun 1, 1984·British Journal of Urology·H HedelinS Pettersson
Jan 1, 1994·Annual Review of Microbiology·J M PattiM Höök
Oct 1, 1993·The Journal of Infectious Diseases·K P McDermidJ W Costerton
Jan 1, 1996·Journal of Bacteriology·J H HeinrichsA L Cheung
Aug 1, 1996·Infection and Immunity·C WolzA L Cheung
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y LuR L Switzer
Mar 1, 1997·FEMS Immunology and Medical Microbiology·B A DegnanJ A Goodacre
Jan 15, 1998·Gastroenterology·D R ScottG Sachs
Nov 27, 1998·Canadian Journal of Microbiology·P C LoewenR Sparling
Sep 28, 1999·Journal of Bacteriology·C Prigent-CombaretP Lejeune
Nov 5, 1999·Trends in Microbiology·M O Clements, S J Foster
Nov 9, 2000·The Journal of Infectious Diseases·C VuongM Otto
Feb 28, 2001·Environmental Microbiology·Y H LiR A Burne
Mar 21, 2001·Antimicrobial Agents and Chemotherapy·K Lewis
Jul 5, 2001·International Journal of Medical Microbiology : IJMM·S Arvidson, K Tegmark

❮ Previous
Next ❯

Citations

Oct 19, 2006·Analytical and Bioanalytical Chemistry·Alexander R HorswillMatthew R Parsek
May 4, 2011·Applied Microbiology and Biotechnology·Esther KarunakaranCatherine A Biggs
Dec 27, 2011·Current Microbiology·Qun ZhangXuemei Zhang
Apr 9, 2010·Medical Microbiology and Immunology·Martin Lappann, Ulrich Vogel
Nov 1, 2008·The Journal of Microbiology·Jingfu QiuRuifu Yang
Apr 2, 2005·Current Opinion in Microbiology·Beth A Lazazzera
Jul 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Medora J HusebyCathleen A Earhart
Apr 12, 2013·Biofouling·M C Ammons, V Copié
Aug 21, 2012·The Journal of Antimicrobial Chemotherapy·Sylvie Buffet-BataillonAnne Jolivet-Gougeon
Aug 5, 2009·Antimicrobial Agents and Chemotherapy·Elizabeth C WeissMark S Smeltzer
Mar 21, 2007·Antimicrobial Agents and Chemotherapy·Naomi BalabanGiorgio Scalise
Apr 9, 2008·Antimicrobial Agents and Chemotherapy·Oriana SimonettiAndrea Giacometti
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·M A Jabra-RizkM E Shirtliff
Nov 7, 2006·Applied and Environmental Microbiology·Peter Sass, Gabriele Bierbaum
May 5, 2005·Applied and Environmental Microbiology·Alexandra ReschFriedrich Götz
Apr 7, 2006·Applied and Environmental Microbiology·Indranil ChatterjeeMathias Herrmann
Feb 14, 2013·Genome Announcements·Janette M HarroMark E Shirtliff
Mar 19, 2008·Infection and Immunity·Kati SeidlMarkus Bischoff
Jan 12, 2011·Infection and Immunity·Rebecca A BradyMark E Shirtliff
Jun 6, 2007·Infection and Immunity·Laura H TsangMark S Smeltzer
Dec 12, 2012·Infection and Immunity·M Indriati HoodEric P Skaar
Feb 14, 2013·Infection and Immunity·Seok-Mo HeoStefan Ruhl
Feb 4, 2009·Infection and Immunity·Katherine J LauderdaleAlexander R Horswill
Dec 13, 2006·Infection and Immunity·Patrick H Tu QuocWilliam L Kelley

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Cellulitis

Cellulitis (erysipelas) is a recurring and debilitating bacterial infection of the skin and underlying tissue. Discover the latest research on cellulitis here.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.