Global gene expression profiles of canine macrophages and canine mammary cancer cells grown as a co-culture in vitro.

BMC Veterinary Research
Magdalena KrólTomasz Motyl

Abstract

Solid tumours comprise various cells, including cancer cells, resident stromal cells, migratory haemopoietic cells and other. These cells regulate tumour growth and metastasis. Macrophages constitute probably the most important element of all interactions within the tumour microenvironment. However, the molecular mechanism, that guides tumour environment, still remains unknown. Exploring the underlying molecular mechanisms that orchestrate these phenomena has been the aim of our study.A co-culture of canine mammary cancer cells and macrophages was established and maintained for 72 hrs. Having sorted the cells, gene expression in cancer cells and macrophages, using DNA microarrays, was examined. The results were confirmed using real-time qPCR and confocal microscopy. Moreover, their ability for migration and invasion has been assessed. Microarray analysis showed that the up-regulated genes in the cancer cell lines are involved in 15 highly over-manifested pathways. The pathways that drew our diligent attention included: the inflammation pathway mediated by chemokine and cytokine, the Toll receptor signalling pathway and the B cell activation. The up-regulated genes in the macrophages were involved in only 18 significantly over-m...Continue Reading

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Citations

Feb 26, 2013·Veterinary Research Communications·K M PawłowskiM Król
Sep 25, 2012·BMC Veterinary Research·Kinga MajchrzakMagdalena Król
Jun 19, 2013·BMC Veterinary Research·Karol M PawłowskiMagdalena Król
Jul 13, 2013·BMC Veterinary Research·Karol M PawłowskiMagdalena Król
Apr 17, 2013·BMC Veterinary Research·Karol M PawłowskiMagdalena Król
Oct 12, 2013·PloS One·Kinga MajchrzakMagdalena Król
Jan 24, 2013·Proteomics. Clinical Applications·Kenneth D ColeLili Wang
Jul 16, 2015·BMC Veterinary Research·Breno C B BeirãoDavid J Argyle
Feb 18, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ernest Giralt, Daniele Lo Re
Jun 28, 2016·Molecular BioSystems·F CecilianiC Lecchi
Apr 30, 2020·Animals : an Open Access Journal From MDPI·Lorena Franco-MartínezAsta Tvarijonaviciute
Jan 6, 2017·BioMed Research International·Maria Isabel CarvalhoFelisbina L Queiroga
Feb 15, 2021·Research in Veterinary Science·Francesca ParisiAlessandro Poli
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Riccardo FinotelloLorenzo Ressel

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Datasets Mentioned

BETA
GSE29339

Methods Mentioned

BETA
FACS
Feature Extraction
Flow cytometry
confocal microscopy
fluorescence microscopy

Software Mentioned

Pathway Studio
GraphPad
Prism
Gene Spring
Fluoview
Feature Extraction
Oligo Calculator
Agilent
- Blast
PRIMER3

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