Global gene expression profiling of Bovine immature B cells using serial analysis of gene expression

Animal Biotechnology
John D NeillElizabeth Liebler-Tenorio

Abstract

The Peyer's patches of the small intestine are important sites of antigen processing. The follicles of the Peyer's patches receive the antigen transported by the M cells at the mucosal surface and then play a major role in development of both protective humoral and mucosal immune responses. Serial analysis of gene expression (SAGE) was employed to derive the global gene expression profile of B lymphocytes isolated from the IPPF. Analysis of the SAGE data revealed the identity of genes and the level to which they are expressed by IPPF B lymphocytes. This analysis indicated that they were metabolically active and that the transcripts encoding proteins necessary for a response to antigen presentation were expressed. These transcripts included the B cell receptor components CD76a and b (Ig-alpha and Ig-beta) and accessory c-Src family kinases Lyn and Blk. Furthermore, many of the positive and negative regulators, and accessory proteins that are necessary for B cell antigen receptor signaling were identified. Also present were transcripts encoding interleukins and their cognate receptors. Overall, this SAGE analysis yielded a global picture of gene expression in IPPF B lymphocytes and provides a starting point for the comparison of ...Continue Reading

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Citations

Oct 18, 2013·The Journal of Veterinary Medical Science·Abdul Qadir QadisShigeru Sato
Aug 9, 2012·Biologicals : Journal of the International Association of Biological Standardization·Ernst Peterhans, Matthias Schweizer
Dec 5, 2017·Cellular & Molecular Immunology·Thaddeus C DeissVaughn V Smider
May 10, 2007·Nature Protocols·Min Hu, Kornelia Polyak

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Datasets Mentioned

BETA
GSM11383

Methods Mentioned

BETA
PCR
flow cytometry

Software Mentioned

SAGE 2000
SAGE

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