Global H3K9 dimethylation status is not affected by transcription, translation, or DNA replication in porcine zygotes

Molecular Reproduction and Development
Ki-Eun ParkRyan A Cabot

Abstract

Methylation of the lysine 9 residue of histone H3 (H3K9) is linked to transcriptional repression. The observed structure of chromatin in porcine and murine embryos is different with regard to H3K9 dimethylation status, leading to our hypothesis that the intracellular mechanisms responsible for H3K9 methylation would also differ between these two species. The objectives of this study were: (1) to determine the extent that DNA, mRNA, and protein synthesis serve in maintaining the asymmetrical distribution of dimethylated H3K9 in porcine zygotes, (2) determine the extent to which the intracellular localization of individual pronuclei correlated with H3K9 dimethylation status, and (3) to determine the abundance of transcripts encoding the histone methyltransferases, with H3K9 methylation activity, in porcine oocytes and embryos. Our findings are that (1) H3K9 dimethylation status is not affected by DNA replication, transcription, or protein synthesis, (2) the location of a pronucleus does not significantly affect the H3K9 dimethylation status of the chromatin within that pronucleus, and (3) the histone methyltransferases with activity for H3K9 differ in transcript abundance in porcine oocytes and cleavage stage embyros. These resul...Continue Reading

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Jun 19, 2009·Molecular Reproduction and Development·Ki-Eun ParkRyan A Cabot

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Citations

Jun 22, 2010·Cell and Tissue Research·Jianguo ZhaoRandall S Prather
Apr 17, 2020·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Xia WuHaiquan Yu
Aug 2, 2017·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xiaowei NieRongfeng Li

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