Oct 7, 2009

Global metabolic profiling of plant cell wall polysaccharide degradation by Saccharophagus degradans

Biotechnology and Bioengineering
Min Hye ShinKyoung Heon Kim

Abstract

Plant cell wall polysaccharides can be used as the main feedstock for the production of biofuels. Saccharophagus degradans 2-40 is considered to be a potent system for the production of sugars from plant biomass due to its high capability to degrade many complex polysaccharides. To understand the degradation metabolism of plant cell wall polysaccharides by S. degradans, the cell growth, enzyme activity profiles, and the metabolite profiles were analyzed by gas chromatography-time of flight mass spectrometry using different carbon sources including cellulose, xylan, glucose, and xylose. The specific activity of cellulase was only found to be significantly higher when cellulose was used as the sole carbon source, but the xylanase activity increased when xylan, xylose, or cellulose was used as the carbon source. In addition, principal component analysis of 98 identified metabolites in S. degradans revealed four distinct groups that differed based on the carbon source used. Furthermore, metabolite profiling showed that the use of cellulose or xylan as polysaccharides led to increased abundances of fatty acids, nucleotides and glucuronic acid compared to the use of glucose or xylose. Finally, intermediates in the pentose phosphate p...Continue Reading

Mentioned in this Paper

Metabolic Process, Cellular
Pentose Phosphate Pathway
Bacterial Proteins
Glucuronic Acid, 6-(14)C-labeled, (D)-isomer
Xylanase Activity
Polysaccharide Catabolic Process
Mass Spectrometry
Cell Wall
Saturated Fat
Enzyme Activity

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.