Global methylation profiles in buccal cells of long-term smokers and moist snuff consumers

Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
Walter J JessenG L Prasad

Abstract

Alternations in gene methylation and other epigenetic changes regulate normal development as well as drive disease progression. The aim of this study is to investigate global methylation changes in the buccal cells of smokers and smokeless tobacco users. Generally healthy adult male subjects were recruited into smoker (SMK), moist snuff consumer (MSC) and non-tobacco consumer (NTC) cohorts (40 subjects/cohort) (ClinicalTrials.gov Identifier: NCT01923402). Global methylation profiling was performed on Illumina 450 K methylation arrays using buccal cell DNAs. The SMK cohort exhibited larger qualitative and quantitative changes relative to MSC. Approximately half of the differentially methylated 1252 gene loci were grouped as combustible tobacco-related (CTR) signatures and a third of the changes, tobacco-related (TR) signatures, were associated with smoking. Very few (41) differentially methylated gene loci were exclusively associated with moist snuff use and were designated as moist snuff-related (MSR) signature. Pathway enrichment analyses revealed that developmental and immune response pathways, among others, were impacted due to tobacco use. Chronic cigarette smoking causes hyper- and hypo-methylation of genes that could cont...Continue Reading

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Mar 2, 2019·Epigenetics : Official Journal of the DNA Methylation Society·Andrei L TurinskyMichael Brudno
Dec 6, 2019·Clinical Epigenetics·Xiaohui WuHuiying Liang

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