Global phosphoproteomic analysis identifies SRMS-regulated secondary signaling intermediates

Proteome Science
Raghuveera K GoelKiven E Lukong

Abstract

The non-receptor tyrosine kinase, SRMS (Src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristoylation sites) is a member of the BRK family kinases (BFKs) which represents an evolutionarily conserved relative of the Src family kinases (SFKs). Tyrosine kinases are known to regulate a number of cellular processes and pathways via phosphorylating substrate proteins directly and/or by partaking in signaling cross-talks leading to the indirect modulation of various signaling intermediates. In a previous study, we profiled the tyrosine-phosphoproteome of SRMS and identified multiple candidate substrates of the kinase. The broader cellular signaling intermediates of SRMS are unknown. In order to uncover the broader SRMS-regulated phosphoproteome and identify the SRMS-regulated indirect signaling intermediates, we performed label-free global phosphoproteomics analysis on cells expressing wild-type SRMS. Using computational database searching and bioinformatics analyses we characterized the dataset. Our analyses identified 60 hyperphosphorylated (phosphoserine/phosphothreonine) proteins mapped from 140 hyperphosphorylated peptides. Bioinfomatics analyses identified a number of significantly enriched biological a...Continue Reading

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Jun 18, 2020·International Journal of Molecular Sciences·Chakia J McClendon, W Todd Miller
May 21, 2020·Molecular & Cellular Proteomics : MCP·Cynthia CheliusMark R Marten
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Cynthia CheliusMark R Marten

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Methods Mentioned

BETA
transfections
fluorescence microscopy
transfection
acetylation

Software Mentioned

Mascot
NetworKIN
Progenesis QI
KinMap
PHOSIDA
Motif
IPA
Ingenuity analysis ( IPA )
R
STRING

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