Jan 1, 2016

Global Prediction of Chromatin Accessibility Using RNA-seq from Small Number of Cells

BioRxiv : the Preprint Server for Biology
Weiqiang ZhouHongkai Ji


Conventional high-throughput technologies for mapping regulatory element activities such as ChIP-seq, DNase-seq and FAIRE-seq cannot analyze samples with small number of cells. The recently developed ATAC-seq allows regulome mapping in small-cell-number samples, but its signal in single cell or samples with ≤500 cells remains discrete or noisy. Compared to these technologies, measuring transcriptome by RNA-seq in single-cell and small-cell-number samples is more mature. Here we show that one can globally predict chromatin accessibility and infer regulome using RNA-seq. Genome-wide chromatin accessibility predicted by RNA-seq from 30 cells is comparable with ATAC-seq from 500 cells. Predictions based on single-cell RNA-seq can more accurately reconstruct bulk chromatin accessibility than using single-cell ATAC-seq by pooling the same number of cells. Integrating ATAC-seq with predictions from RNA-seq increases power of both methods. Thus, transcriptome-based prediction can provide a new tool for decoding gene regulatory programs in small-cell-number samples.

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Mentioned in this Paper

Single-Cell Analysis
Genome-Wide Association Study
Deoxyribonuclease I
Sequence Determinations, RNA
High Throughput Screening
Sequence Determinations
Small Lymphocyte
Chromatin Immunoprecipitation
Genes, Regulator

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