Global profiling of DNA methylation erasure in mouse primordial germ cells.

Genome Research
Sylvain GuibertMichael Weber

Abstract

Epigenetic reprogramming, characterized by loss of cytosine methylation and histone modifications, occurs during mammalian development in primordial germ cells (PGCs), yet the targets and kinetics of this process are poorly characterized. Here we provide a map of cytosine methylation on a large portion of the genome in developing male and female PGCs isolated from mouse embryos. We show that DNA methylation erasure is global and affects genes of various biological functions. We also reveal complex kinetics of demethylation that are initiated at most genes in early PGC precursors around embryonic day 8.0-9.0. In addition, besides intracisternal A-particles (IAPs), we identify rare LTR-ERV1 retroelements and single-copy sequences that resist global methylation erasure in PGCs as well as in preimplantation embryos. Our data provide important insights into the targets and dynamics of DNA methylation reprogramming in mammalian germ cells.

References

Jan 26, 2000·Development, Growth & Differentiation·T YoshimizuY Matsui
Sep 3, 2002·Mechanisms of Development·Petra HajkovaM Azim Surani
Jan 21, 2003·Genesis : the Journal of Genetics and Development·Natasha LaneWolf Reik
Mar 27, 2003·Molecular Reproduction and Development·Shun SatoYasuhisa Matsui
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yukiko YamazakiMarisa S Bartolomei
Nov 5, 2003·Methods : a Companion to Methods in Enzymology·Gordon K Smyth, Terry Speed
Nov 10, 2004·Genomics·Jing-Yu LiColum P Walsh
Jun 7, 2005·Nature·Yasuhide OhinataM Azim Surani
Aug 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yukiko YamazakiRyuzo Yanagimachi
Mar 28, 2006·Nature Cell Biology·Susana GonzaloMaría A Blasco
Mar 17, 2007·Nature Reviews. Genetics·Randy L Jirtle, Michael K Skinner
Jan 17, 2008·Nature Reviews. Genetics·Hiroyuki Sasaki, Yasuhisa Matsui
Jan 22, 2008·Nature Immunology·Elaine Dzierzak, Nancy A Speck
Jul 30, 2008·Current Opinion in Genetics & Development·Nadia C Whitelaw, Emma Whitelaw
Oct 16, 2008·Developmental Cell·Xiajun LiAnne C Ferguson-Smith
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Aug 5, 2010·Nature Reviews. Molecular Cell Biology·Susan C Wu, Yi Zhang
Oct 15, 2010·Nature Biotechnology·Alexander Meissner
Oct 30, 2010·Science·Suhua FengWolf Reik
Nov 9, 2010·Nature Genetics·Julie BorgelMichael Weber
Jun 28, 2011·Nature Genetics·Sébastien A SmallwoodGavin Kelsey
Oct 1, 2009·Epigenomics·Sylvain GuibertMichael Weber

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Citations

May 3, 2013·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Neil A YoungsonEmma Whitelaw
Oct 26, 2012·Journal of Human Genetics·Izabela LaczmanskaMaria M Sasiadek
May 31, 2013·Journal of Human Genetics·Hongbo Zhao, Taiping Chen
Jun 14, 2013·Nature Communications·Haiyun GanFuchou Tang
Mar 7, 2013·Nature Structural & Molecular Biology·Irene Cantone, Amanda G Fisher
Jul 19, 2013·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Rieko IkedaKuniya Abe
Aug 15, 2013·Human Molecular Genetics·Marcy E RichardsonMiles F Wilkinson
Jun 21, 2013·Molecular Human Reproduction·Serge McGrawClaude Robert
Nov 21, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Stefanie SeisenbergerWolf Reik
Nov 21, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Gavin Kelsey, Robert Feil
Nov 21, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Neil A Youngson, Margaret J Morris
Aug 29, 2013·Biochemistry. Biokhimii︠a︡·A Bortvin
Jun 4, 2014·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Miklos Toth
Sep 26, 2013·Briefings in Functional Genomics·Ozren Bogdanović, José Luis Gómez-Skarmeta
Feb 19, 2013·Epigenomics·Boris Novakovic, Richard Saffery
May 2, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Jessica M StringerPatrick Western
Jan 11, 2014·Database : the Journal of Biological Databases and Curation·Hongbo LiuQiong Wu
Oct 15, 2013·Trends in Cell Biology·Francesco M Piccolo, Amanda G Fisher
Sep 21, 2013·Cellular and Molecular Life Sciences : CMLS·James H CrichtonIan R Adams
Apr 23, 2014·Current Opinion in Obstetrics & Gynecology·Asli Uyar, Emre Seli
Sep 17, 2014·Epigenetics & Chromatin·Catherine M RoseAmanda J Drake
Apr 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Danny LeungBing Ren
Aug 1, 2014·Molecular and Cellular Endocrinology·John R McCarrey
Oct 29, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Ufuk GünesdoganM Azim Surani
Oct 29, 2014·The Journal of Endocrinology·K A ChanD M Sloboda
Feb 18, 2016·Cellular and Molecular Life Sciences : CMLS·Hsiao P J Voon, Richard J Gibbons
Feb 5, 2015·International Journal of Environmental Research and Public Health·Francesca PiriniRafael Guerrero-Preston
Sep 10, 2013·Seminars in Nephrology·Mingyu LiangYong Liu
Sep 15, 2015·Epigenomics·Lexie ProkopukJessica M Stringer
Dec 19, 2015·Hormone Molecular Biology and Clinical Investigation·Onder CelikSudenaz Celik
Mar 21, 2013·Current Opinion in Cell Biology·Stefanie SeisenbergerWolf Reik
Nov 26, 2015·Seminars in Cell & Developmental Biology·Kirsten Hogg, Patrick S Western
Aug 16, 2015·Critical Reviews in Oncology/hematology·Agnieszka Anna Rawłuszko-WieczorekPaweł Piotr Jagodziński
Oct 27, 2015·Trends in Biotechnology·Fereshteh EsfandiariHossein Baharvand
Jan 15, 2014·Scientific Reports·Yuki KawasakiFumitoshi Ishino
Dec 18, 2012·The EMBO Journal·Saya KagiwadaMitinori Saitou
Jul 25, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Virginie LecomteMargaret J Morris
Jan 10, 2013·The EMBO Journal·Zachary D Smith, Alexander Meissner

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