Global proteomic analysis of the insoluble, soluble, and supernatant fractions of the psychrophilic archaeon Methanococcoides burtonii. Part II: the effect of different methylated growth substrates

Journal of Proteome Research
Timothy J WilliamsRicardo Cavicchioli

Abstract

Methanococcoides burtonii is a cold-adapted methanogenic archaeon from Ace Lake in Antarctica. Methanol and methylamines are the only substrates it can use for carbon and energy. We carried out quantitative proteomics using iTRAQ of M. burtonii cells grown on different substrates (methanol in defined media or trimethylamine in complex media), using techniques that enriched for secreted and membrane proteins in addition to cytoplasmic proteins. By integrating proteomic data with the complete, manually annotated genome sequence of M. burtonii, we were able to gain new insight into methylotrophic metabolism and the effects of methanol on the cell. Metabolic processing of methanol and methylamines is initiated by methyltransferases specific for each substrate, with multiple paralogs for each of the methyltransferases (similar to other members of the Methanosarcinaceae). In M. burtonii, most methyltransferases appear to have distinct roles in the metabolism of methylated substrates, although two methylamine methyltransferases appear to be nonfunctional. One set of methyltransferases for trimethylamine catabolism appears to be membrane associated, potentially providing a mechanism to directly couple trimethylamine uptake to demethyla...Continue Reading

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H HippeG Gottschalk
Mar 1, 1977·Bacteriological Reviews·R K ThauerK Decker
Jun 1, 1983·Journal of Bacteriology·E BellionJ A Bolbot
Jun 1, 1995·Microbiological Reviews·J SikkemaB Poolman
Jun 27, 1998·Journal of Bacteriology·S A BurkeJ A Krzycki
Jul 22, 1998·Progress in Biophysics and Molecular Biology·C Anthony, M Ghosh
Feb 15, 2001·Frontiers in Bioscience : a Journal and Virtual Library·A J Macario, E Conway De Macario
Apr 5, 2002·Genome Research·James E GalaganBruce Birren
Jul 10, 2002·Progress in Nucleic Acid Research and Molecular Biology·Uwe Deppenmeier
Aug 6, 2002·Research in Microbiology·Yogita Sardessai, Saroj Bhosle
May 7, 2003·Current Opinion in Microbiology·Kristian KvintThomas Nyström
Jul 1, 2004·Molecular Microbiology·Amber GoodchildRicardo Cavicchioli
Sep 24, 2004·Molecular & Cellular Proteomics : MCP·Philip L RossDarryl J Pappin
Feb 15, 2005·Journal of Proteome Research·Qingbo LiJames G Ferry
Apr 5, 2005·Archaea : an International Microbiological Journal·Sebastian BäumerUwe Deppenmeier
Apr 13, 2005·Journal of Proteome Research·Amber GoodchildRicardo Cavicchioli
Dec 20, 2005·Molecular Microbiology·Anirban MahapatraJoseph A Krzycki
May 23, 2006·Nature Reviews. Microbiology·Ricardo Cavicchioli
Jan 24, 2007·Acta Crystallographica. Section D, Biological Crystallography·Jürgen BoschLars-Oliver Essen
Feb 13, 2007·Trends in Biochemical Sciences·Susan C Wang, Perry A Frey
Apr 10, 2007·Journal of Bacteriology·Zalán SzabóArnold J M Driessen
Jun 19, 2007·Archives of Microbiology·Anuradha GhoshRakesh K Jain
Oct 28, 2008·Nature Chemical Biology·Abhishek ChatterjeeSteven E Ealick

❮ Previous
Next ❯

Citations

Mar 29, 2014·Archaea : an International Microbiological Journal·Shemsi DemolliMichael Rother
Nov 5, 2014·Translational Psychiatry·A PoljakG A Caplan
Mar 6, 2012·Current Opinion in Microbiology·Julie A Maupin-FurlowPhillip Aaron Kirkland
Jan 1, 2014·Proteomics·Andreas OttoFrank Schmidt
Sep 18, 2010·Archaea : an International Microbiological Journal·Michael Rother, Joseph A Krzycki
Apr 27, 2011·Environmental Microbiology·Dominic BurgRicardo Cavicchioli
Nov 10, 2010·Environmental Microbiology·S CampanaroR Cavicchioli
May 20, 2015·Cell Stress & Chaperones·C M Santosh KumarGaurang Mahajan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.