Global Regulation of Gene Expression by the MafR Protein of Enterococcus faecalis

Frontiers in Microbiology
Sofía Ruiz-CruzAlicia Bravo

Abstract

Enterococcus faecalis is a natural inhabitant of the human gastrointestinal tract. However, as an opportunistic pathogen, it is able to colonize other host niches and cause life-threatening infections. Its adaptation to new environments involves global changes in gene expression. The EF3013 gene (here named mafR) of E. faecalis strain V583 encodes a protein (MafR, 482 residues) that has sequence similarity to global response regulators of the Mga/AtxA family. The enterococcal OG1RF genome also encodes the MafR protein (gene OG1RF_12293). In this work, we have identified the promoter of the mafR gene using several in vivo approaches. Moreover, we show that MafR influences positively the transcription of many genes on a genome-wide scale. The most significant target genes encode components of PTS-type membrane transporters, components of ABC-type membrane transporters, and proteins involved in the metabolism of carbon sources. Some of these genes were previously reported to be up-regulated during the growth of E. faecalis in blood and/or in human urine. Furthermore, we show that a mafR deletion mutant strain induces a significant lower degree of inflammation in the peritoneal cavity of mice, suggesting that enterococcal cells def...Continue Reading

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Citations

Oct 18, 2016·Frontiers in Molecular Biosciences·Virtu Solano-ColladoAlicia Bravo
Mar 15, 2018·FEBS Letters·Sofía Ruiz-CruzAlicia Bravo
Apr 23, 2019·PLoS Pathogens·Martinna BertoliniAnna Dongari-Bagtzoglou
Apr 18, 2019·Scientific Reports·Sofía Ruiz-CruzAlicia Bravo
Aug 28, 2020·Frontiers in Molecular Biosciences·José Francisco Sanchez-HerreroAntonio Juárez

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Datasets Mentioned

BETA
GSE75409
2
AE016830.1
CP002621.1

Methods Mentioned

BETA
X-ray
PCR
reverse transcription-PCR
electrophoresis
PCRs
flow cytometry
lavage
enzyme-linked immunosorbent assay
RNA-seq

Software Mentioned

bend
needle
Marray
EMBOSS
eArray
Limma
EMA
pcaMethods
. it
Bioconductor

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