Global regulation of the interphase microtubule system by abundantly expressed Op18/stathmin.

Molecular Biology of the Cell
Mikael E SellinM Gullberg

Abstract

Op18/stathmin (Op18), a conserved microtubule-depolymerizing and tubulin heterodimer-binding protein, is a major interphase regulator of tubulin monomer-polymer partitioning in diverse cell types in which Op18 is abundant. Here, we addressed the question of whether the microtubule regulatory function of Op18 includes regulation of tubulin heterodimer synthesis. We used two human cell model systems, K562 and Jurkat, combined with strategies for regulatable overexpression or depletion of Op18. Although Op18 depletion caused extensive overpolymerization and increased microtubule content in both cell types, we did not detect any alteration in polymer stability. Interestingly, however, we found that Op18 mediates positive regulation of tubulin heterodimer content in Jurkat cells, which was not observed in K562 cells. By analysis of cells treated with microtubule-poisoning drugs, we found that Jurkat cells regulate tubulin mRNA levels by a posttranscriptional mechanism similarly to normal primary cells, whereas this mechanism is nonfunctional in K562 cells. We present evidence that Op18 mediates posttranscriptional regulation of tubulin mRNA in Jurkat cells through the same basic autoregulatory mechanism as microtubule-poisoning drug...Continue Reading

References

Feb 1, 1992·Molecular and Cellular Biology·N G Theodorakis, D W Cleveland
Feb 1, 1989·Current Opinion in Cell Biology·D W Cleveland
Nov 1, 1985·The Journal of Cell Biology·J M CaronM W Kirschner
Jun 14, 1996·The Journal of Biological Chemistry·U K SchubartW Edelmann
Dec 1, 1996·The Journal of Cell Biology·M L Gonzalez-Garay, F Cabral
Mar 28, 1997·The Journal of Biological Chemistry·S B HorwitzU K Schubart
Dec 31, 1997·International Review of Cytology·R F Ludueña
Jan 1, 1997·Annual Review of Cell and Developmental Biology·A Desai, T J Mitchison
Mar 27, 1999·The Journal of Biological Chemistry·T NarishigeG Cooper
Feb 17, 2000·The EMBO Journal·M O SteinmetzJ van Oostrum
Jan 17, 2002·Current Opinion in Cell Biology·Lynne Cassimeris
Feb 13, 2002·The American Journal of Pathology·Wolfgang LiedtkeUlrich K Schubart
Oct 23, 2002·Journal of Neuroscience Research·Nozomu Mori, Hiroshi Morii
Nov 16, 2005·Cell·Gleb P ShumyatskyVadim Y Bolshakov
Mar 24, 2006·The Journal of Biological Chemistry·Yaqing WangFernando Cabral
Apr 21, 2006·Molecular Biology of the Cell·Per HolmfeldtMartin Gullberg
Mar 9, 2007·Molecular Biology of the Cell·Per HolmfeldtMartin Gullberg
Jun 9, 2007·Current Biology : CB·Georgina Fletcher, Pernille Rørth

❮ Previous
Next ❯

Citations

Jul 16, 2010·The Journal of Biological Chemistry·Dominic C H NgMarie A Bogoyevitch
Mar 3, 2009·The Journal of Biological Chemistry·Navin K VermaYuri Volkov
Jun 12, 2009·Molecular Biology of the Cell·Danielle N Ringhoff, Lynne Cassimeris
Oct 15, 2011·Molecular Biology of the Cell·Mikael E SellinMartin Gullberg
Sep 8, 2012·Molecular Biology of the Cell·Mikael E SellinMartin Gullberg
Apr 7, 2010·Molecular and Cellular Biology·Mourad SanhajiJuping Yuan
Aug 23, 2012·Annals of Surgical Oncology·Yi-Ling ChenYow-Ling Shiue
Jul 9, 2011·Molecular Biology of the Cell·Mikael E SellinMartin Gullberg
Jul 9, 2009·Cellular and Molecular Life Sciences : CMLS·Per HolmfeldtMartin Gullberg
Feb 2, 2010·Trends in Biochemical Sciences·Victor F LundinPeter C Stirling
Aug 12, 2009·Biochemical and Biophysical Research Communications·Gianni Harris, Katherine L Schaefer
Dec 14, 2016·Journal of the American Chemical Society·Christoffer HamarkGöran Widmalm
Sep 17, 2009·Biochemical Society Transactions·Babet van der VaartAnne Straube
Nov 22, 2013·Biochemical Society Transactions·Jonathan L D Lawson, Rafael E Carazo Salas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.