Global structure of microwave-assisted flash-extracted sugar beet pectin

Journal of Agricultural and Food Chemistry
Marshall L FishmanArland T Hotchkiss

Abstract

The global structure of microwave-assisted flash-extracted pectins isolated from fresh sugar beet pulp has been studied. The objective was to minimize the disassembly and possibly the degradation of pectin molecules during extraction. These pectins have been characterized by high-performance size exclusion chromatography with light scattering, viscometric detection, and atomic force microscopy (AFM). Analysis of molecular parameters was performed on 15 and 8 microm size column packings. Samples analyzed with 15 microm packing gave weight-average molar masses that ranged from 532,000 to 1.2 million Da, radii of gyration from about 35 to 51 nm, polydispersities from 1.78 to 2.58, intrinsic viscosities from about 3.00 to 4.30 dL/g, and recoveries from 8.40 to 14.81% of dry weight. Chromatography revealed that a bimodal distribution of high molar mass spherical particles and lower molar mass coils was obtained. AFM images of pectin corroborated this conclusion and further revealed that these strands and spherical particles were integrated into networks. It is demonstrated that microwave-assisted extraction of sugar beet pulp under moderate pressure and at relatively low temperature could extract under acid conditions high molar mas...Continue Reading

References

Apr 1, 1992·Archives of Biochemistry and Biophysics·M L FishmanR Scorza
Aug 15, 1991·Analytical Biochemistry·T M Filisetti-Cozzi, N C Carpita
Oct 4, 1993·Carbohydrate Research·M L FishmanW Damert
Sep 18, 2001·Journal of Agricultural and Food Chemistry·M L FishmanJ Brady
Dec 4, 2003·Journal of Agricultural and Food Chemistry·Sang-Ho YooArland T Hotchkiss
Mar 9, 2004·Biomacromolecules·Marshall L FishmanDavid R Coffin
Feb 13, 2007·Biomacromolecules·Marshall L FishmanArland T Hotchkiss

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Citations

Jan 24, 2013·Journal of Agricultural and Food Chemistry·Xiangyang LiSaphwan Al-Assaf
May 15, 2015·Journal of Agricultural and Food Chemistry·Arland T HotchkissChristina Khoo
Feb 13, 2013·Carbohydrate Polymers·Marshall L FishmanMadhav P Yadav
May 22, 2013·Carbohydrate Polymers·Ana M M SousaMaria P Gonçalves
May 23, 2017·Analytical Chemistry·Phoonthawee SaetearHervé Cottet
Apr 21, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·A GromerV J Morris
Oct 29, 2009·Journal of Agricultural and Food Chemistry·Alberto NuñezArland T Hotchkiss
Jul 10, 2019·Journal of Agricultural and Food Chemistry·Daiki MurayamaShinya Ikeda

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