Global Survey and Expressions of the Phosphate Transporter Gene Families in Brassica napus and Their Roles in Phosphorus Response

International Journal of Molecular Sciences
Jin YangHai Du

Abstract

Phosphate (Pi) transporters play critical roles in Pi acquisition and homeostasis. However, currently little is known about these genes in oil crops. In this study, we aimed to characterize the five Pi transporter gene families (PHT1-5) in allotetraploid Brassica napus. We identified and characterized 81 putative PHT genes in B. napus (BnaPHTs), including 45 genes in PHT1 family (BnaPHT1s), four BnaPHT2s, 10 BnaPHT3s, 13 BnaPHT4s and nine BnaPHT5s. Phylogenetic analyses showed that the largest PHT1 family could be divided into two groups (Group I and II), while PHT4 may be classified into five, Groups I-V. Gene structure analysis revealed that the exon-intron pattern was conservative within the same family or group. The sequence characteristics of these five families were quite different, which may contribute to their functional divergence. Transcription factor (TF) binding network analyses identified many potential TF binding sites in the promoter regions of candidates, implying their possible regulating patterns. Collinearity analysis demonstrated that most BnaPHTs were derived from an allopolyploidization event (~40.7%) between Brassica rapa and Brassica oleracea ancestors, and small-scale segmental duplication events (~39.5...Continue Reading

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Citations

Mar 27, 2020·International Journal of Molecular Sciences·Yuanyuan WanCunmin Qu

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Datasets Mentioned

BETA
PRJNA358784
PRJNA608211

Methods Mentioned

BETA
RNA-Seq
PCR
electrophoresis

Software Mentioned

FigTree
TMHMM
MAFFT
MEGA5
SMART
Gene Structure Display Server ( GSDS )
R package
MEGA
Mapchart
CoGe

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