Global transcription analysis of immature avian erythrocytic progenitors: from self-renewal to differentiation

Oncogene
Francesca DamiolaOlivier Gandrillon

Abstract

The molecular mechanisms regulating the cell fate decision between self-renewal and differentiation/apoptosis in stem and progenitor cells are poorly understood. Here, we report the first comprehensive identification of genes potentially involved in the switch from self-renewal toward differentiation of primary, non-immortalized erythroid avian progenitor cells (T2EC cells). We used the Serial Analysis of Gene Expression (SAGE) technique in order to identify and quantify the genome fraction functionally active in a self-renewing versus a differentiating cell population. We generated two SAGE libraries and sequenced a total of 37,589 tags, thereby obtaining the first transcriptional profile characterization of a chicken cell. Tag identification was performed using a new relational database (Identitag) developed in the laboratory, which allowed a highly satisfactory level of identification. Among 123 differentially expressed genes, 11 were investigated further and for nine of them the differential expression was subsequently confirmed by real-time PCR. The comparison of tag abundance between the two libraries revealed that only a small fraction of transcripts was differentially expressed. The analysis of their functions argue aga...Continue Reading

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Citations

Sep 20, 2008·BMC Bioinformatics·Johan LeyritzOlivier Gandrillon
Oct 27, 2007·BMC Genomics·Corinne BressonSandrine Gonin-Giraud
May 10, 2007·Nature Protocols·Min Hu, Kornelia Polyak
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Oct 1, 2008·Cell Proliferation·C Bresson-MazetS Gonin-Giraud
Jul 25, 2012·Current Opinion in Pharmacology·Miguel A LasunciónRebeca Busto

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