Global transcriptional analysis of Methanosarcina mazei strain Gö1 under different nitrogen availabilities.

Molecular Genetics and Genomics : MGG
Katharina VeitRuth A Schmitz

Abstract

Certain archaeal species can fix molecular nitrogen under nitrogen limiting conditions although little is known about this process at either the genetic or molecular level. To address this on a genome-wide scale, transcriptional analysis was performed on the model methanogen Methanosarcina mazei strain Gö1 using DNA-microarrays. The genomic expression patterns for cells grown under nitrogen fixing conditions versus nitrogen sufficiency (10 mM ammonium) revealed that approximately 5% of all genes are differentially expressed. Besides a small set of genes previously known to be up-regulated under nitrogen limitation, 14 additional genes involved in nitrogen metabolism were identified plus 10 genes encoding potential transcriptional regulators, 13 genes involved in carbon metabolism, 3 genes in general stress response, 8 putative transporter genes, and an additional 21 genes with unknown function. Quantitative reverse transcriptase PCR experiments confirmed the differential expression of a subset of these genes. Promoter analysis revealed a palindromic DNA motif centered nearby the transcriptional start point for several genes up-regulated under nitrogen limitation. A bioinformatics study demonstrated the presence of this motif in...Continue Reading

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Citations

Apr 2, 2008·Archives of Microbiology·Regina Spanheimer, Volker Müller
Apr 17, 2008·Archives of Microbiology·Katrin WeidenbachRuth A Schmitz
Aug 8, 2009·Applied Microbiology and Biotechnology·Silke AllgaierFrank Kempken
Dec 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Dominik JägerRuth A Schmitz
Sep 12, 2012·Nucleic Acids Research·Dominik JägerRuth A Schmitz
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Sep 8, 2017·Journal of Virology·Katrin WeidenbachRuth A Schmitz
Jan 5, 2017·BMC Evolutionary Biology·Giorgio Matassi
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Aug 23, 2019·Journal of Molecular Biology·Rylee K Hackley, Amy K Schmid
Mar 11, 2021·Proceedings of the National Academy of Sciences of the United States of America·Adrian C D FuchsAndrei N Lupas

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