Global transcriptional analysis of the phosphate starvation response in Sinorhizobium meliloti strains 1021 and 2011

Molecular Genetics and Genomics : MGG
Elizaveta Krol, Anke Becker

Abstract

The global response to phosphate starvation was analysed at the transcriptional level in two closely related strains of Sinorhizobium meliloti, Rm1021 and Rm2011. The Pho regulon is known to be induced by PhoB under conditions of phosphate limitation. Ninety-eight genes were found to be significantly induced (more than three-fold) in a phoB -dependent manner in phosphate-stressed cells, and phoB -independent repression of 86 genes was observed. Possible roles of these genes in the phosphate stress response are discussed. Twenty new putative PHO box sequences were identified in regions upstream of 17 of the transcriptional units that showed phoB -dependent, or partially phoB -dependent, regulation, indicating direct regulation of these genes by PhoB. Despite the overall similarity between the phosphate stress responses in Rm1021 and Rm2011, lower induction rates were found for a set of phoB -dependent genes in Rm1021. Moreover, Rm1021 exhibited moderate constitutive activation of 12 phosphate starvation-inducible, phoB -dependent genes when cells were grown in a complex medium. A 1-bp deletion was observed in the pstC ORF in Rm1021, which results in truncation of the protein product. This mutation is probably responsible for the...Continue Reading

References

May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H M Meade, E R Signer
Sep 1, 1974·Journal of General Microbiology·J E Beringer
Aug 1, 1996·Journal of Bacteriology·R A VanBogelenF C Neidhardt
Apr 8, 1998·Molecular & General Genetics : MGG·B Spira, E Yagil
May 9, 1998·Applied and Environmental Microbiology·X Wei, W D Bauer
Nov 7, 1998·Molecular Plant-microbe Interactions : MPMI·M L SummersT R McDermott
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Mar 27, 1999·Journal of Bacteriology·E SuziedelienéS Normark
Jan 14, 2000·Journal of Bacteriology·K E Mendrygal, J E González
Mar 27, 2001·Nucleic Acids Research·F DiehlJ D Hoheisel
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·R M Klug, C Benning
Jun 16, 2001·Advances in Microbial Physiology·P A Lund
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·T M FinanA Pühler
Dec 17, 2002·Molecular Plant-microbe Interactions : MPMI·Rebecca J WaisSharon R Long
May 3, 2003·The EMBO Journal·Maria Arménia Carrondo
Jun 11, 2003·Molecular Plant-microbe Interactions : MPMI·Michael A DjordjevicUNKNOWN Sinorhizobium DNA Sequencing Consortium
Oct 11, 2003·FEMS Microbiology Reviews·Olga A Soutourina, Philippe N Bertin
Oct 18, 2003·Journal of Bacteriology·Christophe MerlinAndrew Coulson
Dec 4, 2003·Journal of Biotechnology·Michael DondrupFolker Meyer

❮ Previous
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Citations

May 25, 2012·Applied Microbiology and Biotechnology·Juan F MartínEllizabeth M H Wellington
Nov 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Svetlana N Yurgel, Michael L Kahn
Apr 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Alexander ZaborinJohn C Alverdy
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Maritza Zavaleta-PastorOtto Geiger
Apr 20, 2013·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Erika SalletThomas Schiex
May 24, 2006·Nucleic Acids Research·Ze-Chun YuanTurlough M Finan
Aug 4, 2011·Molecular Plant-microbe Interactions : MPMI·Maarten VercruysseJan Michiels
Apr 14, 2006·Molecular Plant-microbe Interactions : MPMI·Delphine CapelaClaude Bruand
Nov 6, 2007·Molecular Plant-microbe Interactions : MPMI·Gabriella PessiHauke Hennecke
Oct 10, 2008·Molecular Plant-microbe Interactions : MPMI·Christelle BahlawaneAnke Becker
Aug 15, 2008·Molecular Plant-microbe Interactions : MPMI·Maria de las Nieves PeltzerDelphine Capela
May 21, 2008·Genome Research·Claire AmadouCatherine Masson-Boivin
Jun 9, 2007·Plant Physiology·Allyson M MacLeanMichael J Sadowsky
Jun 30, 2009·Applied and Environmental Microbiology·Emanuele G BiondiCarlo Viti
Dec 18, 2008·Applied and Environmental Microbiology·Arati V Patankar, Juan E González
Oct 26, 2010·Applied and Environmental Microbiology·Petra R A KohlerSilvia Rossbach
Oct 6, 2005·Applied and Environmental Microbiology·Tzu-Chiao ChaoStefan Weidner
Mar 7, 2006·Applied and Environmental Microbiology·Russell D MondsGeorge A O'Toole
Apr 28, 2009·Journal of Bacteriology·Nataliya Gurich, Juan E González
Aug 19, 2006·Journal of Bacteriology·Ana Domínguez-FerrerasJuan Sanjuán
Jul 24, 2007·Journal of Bacteriology·Sarah A GlennJuan E González
Nov 21, 2007·Journal of Bacteriology·Hanh H HoangJuan E González
Nov 26, 2009·Genome Génome / Conseil National De Recherches Canada·Hong GuoJianping Xu

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