Global transcriptional profiles of Staphylococcus aureus treated with berberine chloride

FEMS Microbiology Letters
Dacheng WangXuming Deng

Abstract

In this study, we show that berberine chloride (BBR) has antimicrobial activities against all 43 tested strains of Staphylococcus aureus, an important human and animal pathogen. However, the response mechanisms of S. aureus to BBR are still poorly understood. Affymetrix GeneChips were used to determine the global transcription of S. aureus triggered by treatment with subinhibitory concentrations of BBR. 468 genes were up-regulated and 262 genes were down-regulated upon exposure to BBR. There was elevated transcription of various transporter genes, including genes involved in multidrug resistance, members of the multidrug and toxin extrusion family, the ferrous iron transporter, the amino acid transporter, the Na(+)/H(+) antiporter, and the potassium cation transporter. Measurements of active transport were used to demonstrate a phenotypic correlation between efflux transporter overexpression and inhibition of BBR uptake. Furthermore, BBR induced the expression of urease genes, sortase enzyme, and iron-regulated surface determinant genes, but repressed transcription of a gene encoding arylamine N-acetyltransferase activity (N315-SA2490). To our knowledge, this is the first analysis of a genome-wide transcription profile of S. au...Continue Reading

References

Jan 1, 1986·Molecular & General Genetics : MGG·P RecseiR P Novick
Sep 1, 1969·Canadian Journal of Microbiology·A H AminK M Abbasi
Jan 1, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G A Ayliffe
May 23, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G L Archer
Jul 27, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J G ChungT F Wang
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·F R StermitzK Lewis
Apr 19, 2000·Antimicrobial Agents and Chemotherapy·G W KaatzT J Foster
Jun 24, 2000·Microbes and Infection·R A Burne, Y Y Chen
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
May 6, 2003·Journal of Bacteriology·Que Chi Truong-BolducDavid C Hooper
Apr 23, 2004·Microbes and Infection·Eric P Skaar, Olaf Schneewind
Jul 3, 2004·The Journal of Antimicrobial Chemotherapy·Glenn W Kaatz, Susan M Seo
Jul 3, 2004·Journal of Bacteriology·Karen E BeenkenMark S Smeltzer
Jul 27, 2004·Antimicrobial Agents and Chemotherapy·Bernd HutterHannes Loferer
Dec 4, 2004·Journal of Bacteriology·Brian WeinrickRichard P Novick
Mar 19, 2005·Journal of Bacteriology·Q C Truong-BolducD C Hooper
Jan 24, 2006·Journal of Bacteriology·Eric P SkaarOlaf Schneewind
Feb 18, 2006·Journal of Bacteriology·Thanh T LuongChia Y Lee
Sep 9, 2006·FEMS Microbiology Letters·Jingfu QiuRuifu Yang
Nov 1, 2006·Molecular Microbiology·Luciano A Marraffini, Olaf Schneewind

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Citations

Jul 8, 2010·The Journal of Antibiotics·Lili R MesakJulian Davies
Sep 21, 2011·The Journal of Antimicrobial Chemotherapy·Tina Wecke, Thorsten Mascher
Jan 30, 2010·International Journal of Molecular Sciences·Rocío González-LamotheKamal Bouarab
Nov 19, 2013·Omics : a Journal of Integrative Biology·Kubra KaraosmanogluBerna Sariyar Akbulut
May 28, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Xiaoqing WangZhen'an Zhu
Aug 22, 2015·Journal of Medical Microbiology·Ganapathy Ashwinkumar SubrameniumShunmugiah Karutha Pandian
Aug 27, 2009·Journal of Biomedicine & Biotechnology·Haihua FengLu Yu
Jun 18, 2016·Journal of Asian Natural Products Research·Wen-Li LiNa Guo
Oct 13, 2016·Frontiers in Microbiology·Bruno S Dos SantosPatrícia M G Paiva
Mar 31, 2018·Frontiers in Microbiology·Jill S McClary, Alexandria B Boehm
May 16, 2020·Frontiers in Microbiology·Xiujuan ZhangXiaoyuan Wang
Dec 12, 2020·International Immunopharmacology·Jun-Xiang ShuXian-Hui He
Oct 4, 2020·Microbial Pathogenesis·Cemile Selin AksoyBerna Sariyar Akbulut

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