Global Transcriptional Response to Organic Hydroperoxide and the Role of OhrR in the Control of Virulence Traits in Chromobacterium violaceum

Infection and Immunity
Maristela Previato-MelloJosé F da Silva Neto

Abstract

A major pathway for the detoxification of organic hydroperoxides, such as cumene hydroperoxide (CHP), involves the MarR family transcriptional regulator OhrR and the peroxidase OhrA. However, the effect of these peroxides on the global transcriptome and the contribution of the OhrA/OhrR system to bacterial virulence remain poorly explored. Here, we analyzed the transcriptome profiles of Chromobacterium violaceum exposed to CHP and after the deletion of ohrR, and we show that OhrR controls the virulence of this human opportunistic pathogen. DNA microarray and Northern blot analyses of CHP-treated cells revealed the upregulation of genes related to the detoxification of peroxides (antioxidant enzymes and thiol-reducing systems), the degradation of the aromatic moiety of CHP (oxygenases), and protection against other secondary stresses (DNA repair, heat shock, iron limitation, and nitrogen starvation responses). Furthermore, we identified two upregulated genes (ohrA and a putative diguanylate cyclase with a GGDEF domain for cyclic di-GMP [c-di-GMP] synthesis) and three downregulated genes (hemolysin, chitinase, and collagenase) in the ohrR mutant by transcriptome analysis. Importantly, we show that OhrR directly repressed the expr...Continue Reading

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Citations

Nov 28, 2017·Frontiers in Microbiology·Juliana H Batista, José F da Silva Neto
Aug 4, 2018·Antioxidants & Redox Signaling·Emma SevillaMaría F Fillat
Nov 30, 2018·Microbiology and Molecular Biology Reviews : MMBR·Ashish GuptaAnne Grove
Aug 30, 2020·Applied and Environmental Microbiology·Renato Elias Rodrigues de Souza SantosJosé Freire da Silva Neto

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