Global transcriptome analysis identifies weight regain-induced activation of adaptive immune responses in white adipose tissue of mice

International Journal of Obesity : Journal of the International Association for the Study of Obesity
D S KyungJ K Seong

Abstract

Studies have indicated that weight regain following weight loss predisposes obese individuals to metabolic disorders; however, the molecular mechanism of this potential adverse effect of weight regain is not fully understood. Here we investigated global transcriptome changes and the immune response in mouse white adipose tissue caused by weight regain. We established a diet switch protocol to compare the effects of weight regain with those of weight gain without precedent weight loss, weight loss maintenance and chow diet. We conducted a time course analysis of global transcriptome changes in gonadal white adipose tissue (gWAT) during the weight fluctuation. Co-expression network analysis was used to identify functional modules associated with the weigh regain phenotype. Immune cell populations in gWAT were characterized by flow-cytometric immunophenotyping. Metabolic phenotypes were monitored by histological analysis of adipose tissue and liver, and blood-chemistry and body weight/composition analyses. In total, 952 genes were differentially expressed in the gWAT in the weight regain vs the weight gain group. Upregulated genes were associated with immune response and leukocyte activation. Co-expression network analysis showed ...Continue Reading

References

May 1, 1989·The American Journal of Clinical Nutrition·G L BlackburnK D Brownell
Mar 28, 2002·Nucleic Acids Research·A J EnrightC A Ouzounis
May 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Terry J Fry, Crystal L Mackall
Feb 4, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Paul S MacLeanJames O Hill
Jan 4, 2007·The Journal of Clinical Investigation·Carey N LumengAlan R Saltiel
Sep 13, 2007·Diabetes·Katherine J StrisselMartin S Obin
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Dec 31, 2008·BMC Bioinformatics·Peter Langfelder, Steve Horvath
May 19, 2010·International Journal of Obesity : Journal of the International Association for the Study of Obesity·J L KraschnewskiC N Sciamanna
May 19, 2011·Current Opinion in Clinical Nutrition and Metabolic Care·David L MorrisCarey N Lumeng
Jun 3, 2011·The Journal of Clinical Investigation·Kai SunPhilipp E Scherer
Jun 17, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Paul S MacleanMatthew R Jackman
Aug 1, 2012·PloS One·Sandra Barbosa-da-SilvaMarcia B Aguila
Sep 18, 2014·The Journal of Endocrinology·Mark A ExleyLydia Lynch
Sep 30, 2014·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·T MehtaK Casazza
Jan 24, 2015·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·P S MacLeanM R Jackman
Apr 22, 2015·International Journal of Obesity : Journal of the International Association for the Study of Obesity·F L Greenway
Aug 8, 2015·International Journal of Obesity : Journal of the International Association for the Study of Obesity·L XiaoX Li

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Citations

May 23, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matthew A CottamAlyssa H Hasty
Apr 10, 2019·American Journal of Physiology. Endocrinology and Metabolism·Michal Kasher-MeronJeffery E Pessin
Dec 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wen ZhangZuisu Yang
Apr 3, 2020·Immunological Reviews·Heather L CaslinAlyssa H Hasty
Apr 30, 2020·Cellular & Molecular Immunology·Minli SunZhaoyu Lin

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Datasets Mentioned

BETA
GSE73345

Methods Mentioned

BETA
RNAseq
fluorescence-activated cell sorting
FACS
PCR
flow cytometry
GTPase

Software Mentioned

ImageJ
FlowJo
GeneAnalytics
R
EdgeR
TopHat
Cytoscape
Databases for functional Annotation , Visualization , and Inte...
flashClust
DAVID

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