Global Transcriptomic Effects of Environmentally Relevant Concentrations of the Neonicotinoids Clothianidin, Imidacloprid, and Thiamethoxam in the Brain of Honey Bees ( Apis mellifera)

Environmental Science & Technology
Verena ChristenKarl Fent

Abstract

Neonicotinoids are implicated in the decline of honey bees, but the molecular basis underlying adverse effects is poorly known. Here we describe global transcriptomic profiles in the brain of honey bee workers exposed for 48 h at one environmentally realistic and one sublethal concentration of 0.3 and 3.0 ng/bee clothianidin and imidacloprid, respectively, and 0.1 and 1.0 ng/bee thiamethoxam (1-30 ng/mL sucrose solution) by high-throughput RNA-sequencing (RNA-seq). All neonicotinoids led to significant alteration (mainly down-regulation) of gene expression, generally with a concentration-dependent effect. Among many others, genes related to metabolism and detoxification were differently expressed. Gene ontology (GO) enrichment analysis of biological processes revealed catabolic carbohydrate metabolism (regulation of enzyme activities such as amylase), lipid metabolism, and transport mechanisms as shared terms between all neonicotinoids at high concentrations. KEGG pathway analysis indicated that at least two neonicotinoids induced changes in expression of various metabolic pathways: pentose phosphate pathways, starch and sucrose metabolism, and sulfur metabolism, in which glucose 1-dehydrogenase and alpha-amylase were down-regu...Continue Reading

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Citations

Jun 9, 2020·Frontiers in Microbiology·Shimei PangShaohua Chen
May 7, 2021·The Science of the Total Environment·Angela MinnameyerLars Straub
Aug 10, 2021·ACS Sensors·Zachary T JohnsonJonathan C Claussen
Mar 1, 2019·Environmental Science & Technology·Jana AsselmanKarel A C De Schamphelaere
Jun 11, 2019·Journal of Agricultural and Food Chemistry·Guoqin GeRuyan Hou
Aug 31, 2021·The Science of the Total Environment·Yuxue ZhangXianyong Lin

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