Global transcriptomic response of Pseudomonas aeruginosa to chlorhexidine diacetate

Environmental Science & Technology
Chantal W NdeWilliam E Bentley

Abstract

Pseudomonas aeruginosa is implicated in nosocomial infections and chronic respiratory infections in cystic fibrosis patients. Chlorhexidine diacetate (CHX) is a biguanide disinfectant used for bacterial control in the hospital and agricultural and domestic environments. A better understanding of the mechanism of action of CHX and the resulting response elicited by P. aeruginosa to CHX will facilitate its effective utilization for P. aeruginosa control in these environments. This study presents, for the first time, the transcriptomic response of P. aeruginosa to 0.008 mM CHX after 10 and 60 min. Our results reveal that, after both treatment times, membrane transport, oxidative phosphorylation, and electron transport genes were downregulated. After 10 min, DNA repair was downregulated and the oprH gene that blocks the self-promoted uptake of antimicrobials was upregulated. After 60 min, outer membrane protein, flagellum, pilus, oxidative phosphorylation, and electron transport genes were downregulated. The mexC and mexD genes of the MexCD-OprJ multidrug efflux pump were significantly upregulated after both treatment times. The results of this study improve our understanding of the mode of action of CHX on P. aeruginosa and provid...Continue Reading

References

Feb 1, 1992·Journal of Bacteriology·S L SullivanM E Gottesman
Dec 1, 1988·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R E Hancock, A Bell
Apr 1, 1987·Antimicrobial Agents and Chemotherapy·K HiraiS Mitsuhashi
Sep 1, 1984·Reviews of Infectious Diseases·A J Morrison, R P Wenzel
Nov 1, 1983·Journal of the American Academy of Dermatology·J E Sebben
Mar 1, 1995·Antimicrobial Agents and Chemotherapy·N MasudaS Ohya
Mar 6, 1998·Scientific American·S B Levy
Dec 29, 1998·Emerging Infectious Diseases·C Van Delden, B H Iglewski
Jan 9, 1999·Clinical Microbiology Reviews·G McDonnell, A D Russell
Jun 23, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R K Chakraverty, I D Hickson
Feb 8, 2000·The Journal of Antimicrobial Chemotherapy·U TattawasartA D Russell
May 29, 2000·Current Microbiology·V H Varel, D N Miller
Aug 10, 2000·Molecular Microbiology·E H Manting, A J Driessen
Aug 10, 2000·The Journal of Pharmacy and Pharmacology·S N Ostad, P R Gard
Nov 25, 2000·International Journal of Antimicrobial Agents·U TattawasartA D Russell
Mar 10, 2001·Journal of Medical Microbiology·L V da Silva FilhoS R da Silvo Ramos
Apr 11, 2001·Emerging Infectious Diseases·R P Wenzel, M B Edmond
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·J Courcelle, P C Hanawalt
Mar 26, 2003·The Journal of Antimicrobial Chemotherapy·Yuji MoritaTomofusa Tsuchiya
Jun 10, 2003·The Journal of Biological Chemistry·Eunjoo Choi-Rhee, John E Cronan
Aug 30, 2003·Trends in Cell Biology·Rakesh R KhakharSusan M Gasser
May 26, 2005·The Journal of Antimicrobial Chemotherapy·Keith Poole
Aug 30, 2005·Environmental Science & Technology·Wook ChangWilliam E Bentley
Sep 16, 2005·Journal of Applied Microbiology·P Gilbert, L E Moore

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Citations

Mar 2, 2011·Antimicrobial Agents and Chemotherapy·Tom CoenyeEshwar Mahenthiralingam
Nov 28, 2013·Proceedings of the National Academy of Sciences of the United States of America·Karl A HassanIan T Paulsen
Feb 5, 2011·Biochimica Et Biophysica Acta·Myriam OuberaiMarie-Paule Mingeot-Leclercq
Mar 20, 2015·Clinical Microbiology Reviews·Xian-Zhi LiHiroshi Nikaido
May 10, 2020·Applied and Environmental Microbiology·Beatriz Merchel Piovesan PereiraIlias Tagkopoulos

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