Global translation during early development depends on the essential transcription factor PRDM10.

Nature Communications
Brenda Y HanErnesto Guccione

Abstract

Members of the PR/SET domain-containing (PRDM) family of zinc finger transcriptional regulators play diverse developmental roles. PRDM10 is a yet uncharacterized family member, and its function in vivo is unknown. Here, we report an essential requirement for PRDM10 in pre-implantation embryos and embryonic stem cells (mESCs), where loss of PRDM10 results in severe cell growth inhibition. Detailed genomic and biochemical analyses reveal that PRDM10 functions as a sequence-specific transcription factor. We identify Eif3b, which encodes a core component of the eukaryotic translation initiation factor 3 (eIF3) complex, as a key downstream target, and demonstrate that growth inhibition in PRDM10-deficient mESCs is in part mediated through EIF3B-dependent effects on global translation. Our work elucidates the molecular function of PRDM10 in maintaining global translation, establishes its essential role in early embryonic development and mESC homeostasis, and offers insights into the functional repertoire of PRDMs as well as the transcriptional mechanisms regulating translation.

References

Jun 1, 2002·Journal of Biochemistry·Rimiko Koyanagi-KatsutaKazuhisa Sekimizu
Aug 15, 2002·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Donald A SiegelShannon F Becker
May 31, 2006·PLoS Computational Biology·Christine Vogel, Cyrus Chothia
Aug 22, 2006·Trends in Biochemical Sciences·Alan G Hinnebusch
Jun 22, 2007·The EMBO Journal·Mamiko MasutaniHiroaki Imataka
Jan 20, 2010·Nature Immunology·Shane CrottyStephen P Schoenberger
Feb 6, 2010·BMB Reports·Jin-Ah Park, Keun Cheol Kim
Jan 12, 2011·Nature Biotechnology·James T RobinsonJill P Mesirov
Feb 24, 2011·Blood·Francesca AguiloHans-Willem Snoeck
Oct 27, 2011·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Cathrine K FogAnders H Lund
Jun 7, 2012·Development·Tobias Hohenauer, Adrian W Moore
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Nov 30, 2012·Nucleic Acids Research·Kate R RosenbloomW James Kent
Dec 12, 2012·Cell Research·Jia-Chi Yeo, Huck-Hui Ng
Jan 5, 2014·Nature Protocols·Simone PicelliRickard Sandberg
Mar 29, 2014·Nature·Robert A J SignerSean J Morrison
Oct 11, 2014·Cell·Michael BuszczakSean J Morrison
Dec 18, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jakob HofvanderFredrik Mertens
Oct 8, 2015·Genes & Development·Kwon Tae YouV Narry Kim
Nov 13, 2015·Molecular Biology and Evolution·Michel VervoortPierre Kerner
May 7, 2016·Current Opinion in Genetics & Development·Slim MzoughiErnesto Guccione
Jul 12, 2016·The FEBS Journal·Hyun Ju LeeDavid Vilchez
Sep 15, 2016·Nature·Mary E DickinsonStephen A Murray
Oct 6, 2017·Nucleic Acids Research·Leoš Shivaya ValášekStanislava Gunišová
Dec 23, 2017·Nature Cell Biology
Jan 31, 2019·Frontiers in Genetics·Soroush TahmasebiNahum Sonenberg
Feb 14, 2019·Nature Communications·M O ArcherV Angelopoulos
Jul 18, 2019·The Journal of Pathology·Jakob HofvanderFredrik Mertens

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Citations

Mar 29, 2021·The FEBS Journal·Federico Di TullioErnesto Guccione
Sep 26, 2021·Clinical and Experimental Immunology·Jiantao HanXingwang Xie

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Methods Mentioned

BETA
immunoprecipitation
ChIP-seq
RNA-seq
gene knockout
transgenic
genotyping
PCR
transfections
transfection
fluorescence-activated cell sorting

Software Mentioned

Excel
Metascape
R package
Image Lab
Picard
Ensembl
RSEM
Fiji
Integrative Genomics Viewer IGV
annoPeaks

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