Global translational reprogramming is a fundamental layer of immune regulation in plants

Nature
Guoyong XuXinnian Dong

Abstract

In the absence of specialized immune cells, the need for plants to reprogram transcription to transition from growth-related activities to defence is well understood. However, little is known about translational changes that occur during immune induction. Using ribosome footprinting, here we perform global translatome profiling on Arabidopsis exposed to the microbe-associated molecular pattern elf18. We find that during this pattern-triggered immunity, translation is tightly regulated and poorly correlated with transcription. Identification of genes with altered translational efficiency leads to the discovery of novel regulators of this immune response. Further investigation of these genes shows that messenger RNA sequence features are major determinants of the observed translational efficiency changes. In the 5' leader sequences of transcripts with increased translational efficiency, we find a highly enriched messenger RNA consensus sequence, R-motif, consisting of mostly purines. We show that R-motif regulates translation in response to pattern-triggered immunity induction through interaction with poly(A)-binding proteins. Therefore, this study provides not only strong evidence, but also a molecular mechanism, for global tran...Continue Reading

References

Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Feb 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jose M AlonsoJoseph R Ecker
Oct 14, 2003·Plant Physiology·Mark D Curtis, Ueli Grossniklaus
Sep 13, 2005·Annual Review of Microbiology·Alan G Hinnebusch
Aug 30, 2008·The Journal of General Virology·Philippe J DufresneJean-François Laliberté
May 22, 2009·Nucleic Acids Research·Timothy L BaileyWilliam S Noble
Jul 10, 2009·Methods in Molecular Biology·Angelika MustrophJulia Bailey-Serres
Jan 17, 2012·Current Biology : CB·Karolina M Pajerowska-MukhtarXinnian Dong
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Apr 24, 2012·Cell Host & Microbe·Tiffany L DunbarEmily R Troemel
Mar 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Cyril Zipfel
Aug 21, 2013·PLoS Genetics·Cristina BarbosaLuísa Romão
Nov 2, 2013·The Plant Cell·Ming-Jung LiuShu-Hsing Wu
Nov 26, 2013·Plant Science : an International Journal of Experimental Plant Biology·Albrecht G von ArnimJustin N Vaughn
Dec 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Piyada JuntawongJulia Bailey-Serres
Apr 30, 2014·Molecular Plant·Bethany HuotSheng Yang He
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Oct 10, 2014·Genetics and Molecular Research : GMR·J T LiH Y Pan
Dec 3, 2014·Plant Science : an International Journal of Experimental Plant Biology·Shujing WuPing He
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Nov 17, 2015·The Plant Journal : for Cell and Molecular Biology·Lei LeiJinsheng Lai
Jun 18, 2016·Science·Alan G HinnebuschNahum Sonenberg
Aug 2, 2016·Nature Reviews. Immunology·Daniel Couto, Cyril Zipfel

❮ Previous
Next ❯

Citations

Aug 2, 2017·Proteomics·Polly Yingshan Hsu, Philip N Benfey
Nov 22, 2018·Journal of Experimental Botany·Pin-Yao HuangLaurent Zimmerli
Nov 2, 2018·Wiley Interdisciplinary Reviews. RNA·Nathan MeadeDerek Walsh
Jul 25, 2018·Cold Spring Harbor Perspectives in Biology·Nicholas T IngoliaJonathan S Weissman
Apr 21, 2019·Plant & Cell Physiology·Tomoya FujitaShintaro Iwasaki
Jul 16, 2019·Molecular Plant Pathology·Peter Balint-Kurti
Dec 5, 2019·The Plant Journal : for Cell and Molecular Biology·Sharon B GraySiobhan M Brady
Nov 27, 2019·International Journal of Molecular Sciences·Camila C FilgueirasDenis S Willett
Jan 14, 2020·The EMBO Journal·Eleazar RodriguezMorten Petersen
Mar 14, 2020·Database : the Journal of Biological Databases and Curation·Ruixia NiuGuoyong Xu
Nov 26, 2019·Current Protocols in Molecular Biology·Sonia ChothaniOwen J L Rackham
Mar 24, 2020·The Plant Journal : for Cell and Molecular Biology·Lyza Maron
Nov 22, 2019·Bioinformatics·Saket ChoudharyAndrew D Smith
May 30, 2020·Journal of Cell Science·Stephen DiGiuseppeDerek Walsh
Sep 23, 2020·PloS One·Sharifa G CrandallDenis S Willett
Jun 20, 2018·Proceedings of the National Academy of Sciences of the United States of America·Yukio KuriharaMinami Matsui
Jun 19, 2019·The New Phytologist·Yusuke Saijo, Eliza Po-Iian Loo
Aug 21, 2019·Communications Biology·Xiaoyu LiuKarolina M Pajerowska-Mukhtar
Mar 10, 2018·Frontiers in Plant Science·Francisco X NascimentoBernard R Glick
May 18, 2017·Nature·Julia Bailey-Serres, Wenbo Ma
Jul 18, 2018·Frontiers in Plant Science·Serina M Mazzoni-Putman, Anna N Stepanova
May 28, 2020·Journal of Experimental Botany·Udaykumar KageKemal Kazan
Apr 26, 2020·Molecular Genetics and Genomics : MGG·Ana Paula Zotta MotaMaria Fatima Grossi-de-Sa
May 6, 2020·Wiley Interdisciplinary Reviews. RNA·Ricardo A Urquidi CamachoAlbrecht G von Arnim
Jan 10, 2018·Frontiers in Plant Science·Ekaterina V SheshukovaYuri L Dorokhov
Aug 21, 2020·The Journal of Biological Chemistry·Adam R BenthamMark J Banfield
Dec 20, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sascha G KellerYasuteru Urano
Dec 31, 2020·Cell Host & Microbe·Christian FröschelWolfgang Dröge-Laser

❮ Previous
Next ❯

Datasets Mentioned

BETA
U10212

Methods Mentioned

BETA
transgenic
RNA-seq
PCR
Ribo-seq
co-IP
pull down
confocal microscopy

Software Mentioned

MEME
Mock
BioAnalyzer
DESeq2
elf18
Bowtie2

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.