PMID: 11604159Oct 18, 2001Paper

Globotriaosylceramide (Gb(3)/CD77) is synthesized and surface expressed by bovine lymphocytes upon activation in vitro

Veterinary Immunology and Immunopathology
Christian MengeG Baljer

Abstract

Neutral glycosphingolipids (GSLs) are considered activation markers on human lymphocytes, which are fundamental for studying the immune system. For cattle, only a limited number of activation markers has yet been identified. We recently showed that Shiga toxin 1, known to use globotriaosylceramide (Gb(3) syn. CD77) as a cellular receptor, depresses proliferation of activated bovine lymphocytes [Infect. Immunol. 67 (1999b) 2209]. In order to confirm the expression of Gb(3)/CD77 on bovine lymphocytes, we flowcytometrically examined a bovine B-lymphoma cell line (BL-3) and bovine peripheral blood mononuclear cells (PBMC) before and after mitogenic stimulation and biochemically characterized neutral GSLs extracted from PBMC. CD77 was detected on the surface of BL-3 cells and cultured PBMC essentially after mitogenic stimulation. Although expressed by all PBMC subpopulations identified, the portion of CD7+ cells was highest for BoCD8+ cells, followed by B-cells and BoCD4+ cells at day 4 of cultivation. Ceramide trihexoside of stimulated PBMC was structurally determined as Gal(alpha1-4)Gal(1-4)Glc(1-1)ceramide (Gb(3)). Biochemically, Gb(3) was also detected within unstimulated PBMC which contained ceramide monohexoside (CMH) and Gb(3...Continue Reading

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Citations

Aug 4, 2006·Veterinary Immunology and Immunopathology·Christian MengeGeorg Baljer
Oct 26, 2002·International Journal of Medical Microbiology : IJMM·David G E SmithDavid L Gally
Dec 24, 2002·Microbial Pathogenesis·Ivonne StammCh Menge
Feb 15, 2019·Frontiers in Immunology·Tao ZhangRobbert M Spaapen
Apr 25, 2015·Innate Immunity·Christian MengeGeorg Baljer
Aug 2, 2006·Veterinary Immunology and Immunopathology·Etienne MoussayChristian Menge

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