Glomerular dysfunction in the aging Fischer 344 rat is associated with excessive growth and normal mesangial cell function

The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
G F McDermottC I Whiteside

Abstract

Fischer 344 (F344) rats display focal and diffuse glomerulosclerosis with aging postulated to result from loss of normal mesangial cell intrinsic function, e.g., vasoactive hormone signaling, or preservation of normal responsiveness to extrinsic growth factors. In 3-, 17-, and 24-month-old F344 male rats, glomerular structure, measured by PC-based morphometry, and function were compared. Immunoperoxidase staining of glomerular proliferating cell nuclear antigen (PCNA) detected cellular proliferation. Primary cultured mesangial cells from the 3 age groups were studied in parallel. Calcium (Ca2+) signaling, measured by Fura-2 fluorescence, contraction to vasopressin (AVP) 1 microM, measured by videomicroscopy, and proliferative response to platelet-derived growth factor-beta beta (PDGF) were compared. Proteinuria was 13 +/- 4, 38 +/- 17, and 110 +/- 35 mg/24 hours at 3, 17, and 24 months, respectively (n = 5, mean +/- SE, p < .01, 3 vs 24 months), with no change in 24-hour creatinine clearances. Glomerular volumes (n = 200/group) for 3, 17, and 24 months, respectively, were .30 +/- .01, .60 +/- .02, .74 +/- 0.2 x 10(6) micron3 (p < .001, 3 months vs 17 months, and 17 vs 24 months). Glomerular basement membrane (GBM) widths and fr...Continue Reading

Citations

Jul 1, 1998·Age·H Otani, M Mune
Feb 20, 2014·Experimental Gerontology·Christy C BridgesRudolfs K Zalups
May 3, 2016·Mechanisms of Ageing and Development·Elizabeth A Grunz-BorgmannAlan R Parrish
Jan 4, 2007·The Journal of Pathology·J E Martin, M T Sheaff
Sep 1, 2005·International Urology and Nephrology·Fred G Silva
Jun 5, 2002·Physiological Genomics·Chana YagilYoram Yagil

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.