GLP-1 contributes to increases in PGC-1α expression by downregulating miR-23a to reduce apoptosis

Biochemical and Biophysical Research Communications
Chi WangJinchao Zhang

Abstract

GLP-1 can help to overcome problems of liver cells metabolism, not only pancreatic cell. But the explicit mechanism of this effect remains unclear. In recent years, microRNAs have received the attention of researchers and some microRNAs have important implications for diabetes. The mitochondrial protective gene PGC-1α is also closely related to diabetes, and UCP2 is related to anti-mitochondrial oxidative stress, but the mechanism of action of these genes is unclear. In this study, we used HepG2 cell line and used the cell counting kit (CCK) to measure the cell viability with GLP-1(7-36) and/or glucotoxicity. To investigate alterations in gene expression resulting from incubation with GLP-1 (7-36) or hyperglycaemia, the RNA expression levels of miR-23a, PGC-1α, Bak, Bax and UCP2 were quantified using real-time PCR. The protein levels of PGC-1α were determined by western blot. The role of miR-23a in the regulation of PGC-1α was further assessed through cell transfection to downregulate of miR-23a expression. In this study, the viability of HepG2 hepatocytes was decreased under hyperglycaemia, but incubation with 10 nmol/L GLP-1 (7-36) amide for 24 h significantly increased the viability and decreased the mRNA expression levels o...Continue Reading

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Citations

Dec 12, 2018·Frontiers in Endocrinology·Jordan RowlandsRodrigo Carlessi
Mar 29, 2019·Journal of Diabetes Research·Mohammad Sarif MohiuddinHideki Kamiya
Jul 10, 2021·Molecular and Cellular Endocrinology·Yurong Zheng, Antoine E Karnoub
Dec 12, 2021·Acta Pharmacologica Sinica·Lin RuJun-Qi Niu

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