Abstract
GLP-1 can help to overcome problems of liver cells metabolism, not only pancreatic cell. But the explicit mechanism of this effect remains unclear. In recent years, microRNAs have received the attention of researchers and some microRNAs have important implications for diabetes. The mitochondrial protective gene PGC-1α is also closely related to diabetes, and UCP2 is related to anti-mitochondrial oxidative stress, but the mechanism of action of these genes is unclear. In this study, we used HepG2 cell line and used the cell counting kit (CCK) to measure the cell viability with GLP-1(7-36) and/or glucotoxicity. To investigate alterations in gene expression resulting from incubation with GLP-1 (7-36) or hyperglycaemia, the RNA expression levels of miR-23a, PGC-1α, Bak, Bax and UCP2 were quantified using real-time PCR. The protein levels of PGC-1α were determined by western blot. The role of miR-23a in the regulation of PGC-1α was further assessed through cell transfection to downregulate of miR-23a expression. In this study, the viability of HepG2 hepatocytes was decreased under hyperglycaemia, but incubation with 10 nmol/L GLP-1 (7-36) amide for 24 h significantly increased the viability and decreased the mRNA expression levels o...Continue Reading
References
Apr 15, 2000·The Journal of Biological Chemistry·K NakajimaK Hashizume
Jul 26, 2000·The Journal of Cell Biology·S GhatanR S Morrison
Feb 10, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Masayoshi HorimotoGyörgy Baffy
Apr 20, 2004·Diabetologia·J ButeauM Prentki
Jan 22, 2005·Science·Bradford B Lowell, Gerald I Shulman
Jun 23, 2009·Apoptosis : an International Journal on Programmed Cell Death·Jörn M Schattenberg, Marcus Schuchmann
Sep 17, 2009·Apoptosis : an International Journal on Programmed Cell Death·Carla J H van der KallenCasper G Schalkwijk
Nov 7, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Karthikeyan ChandrasekaranAparajita Dey
Jan 25, 2011·Regulatory Peptides·Eva TomasJoel F Habener
Mar 29, 2011·Nature Cell Biology·Sabine D JordanJens C Brüning
Feb 1, 2012·PloS One·Sanming DengYunjie Wang
Sep 11, 2012·The American Journal of Pathology·Nitika A GuptaAllan Kirk
Sep 15, 2012·Neurobiology of Disease·Aaron P RussellTakayuki Akimoto
May 23, 2013·PloS One·Takanobu ShimasakiHironobu Yoshimatsu
May 28, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Akio MonjiToyoaki Murohara
Oct 15, 2013·Gene·Xueling Li
Nov 20, 2013·Biochimica Et Biophysica Acta·Rachel Blake, Ian A Trounce
Mar 8, 2014·Surgery for Obesity and Related Diseases : Official Journal of the American Society for Bariatric Surgery·Adel Pezeshki, Prasanth K Chelikani
Sep 27, 2014·Best Practice & Research. Clinical Endocrinology & Metabolism·Chitoku Toda, Sabrina Diano
Feb 24, 2015·Cell Death and Differentiation·M F JonesA Lal
Citations
May 29, 2016·Basic Research in Cardiology·Daniel J SassoonKieren J Mather
Jun 23, 2020·Journal of Biochemical and Molecular Toxicology·Omnia S El-DeebRasha O Elesawy
Mar 1, 2018·Molecular Medicine Reports·Shengli LiQianmei Sun
Jul 10, 2018·Physiological Reports·Rebecca L ScalzoJane E B Reusch
Nov 9, 2018·Scientific Reports·M Pilar ValdecantosÁngela M Valverde
Aug 4, 2019·Stem Cell Research & Therapy·Fan YangXiaobo Zhang
Dec 12, 2018·Frontiers in Endocrinology·Jordan RowlandsRodrigo Carlessi
Mar 29, 2019·Journal of Diabetes Research·Mohammad Sarif MohiuddinHideki Kamiya
Aug 14, 2020·International Journal of Molecular Sciences·Motoyasu KojimaKeizo Anzai
Oct 4, 2020·Metabolites·Rosaria Vincenza GiglioManfredi Rizzo
Jul 10, 2021·Molecular and Cellular Endocrinology·Yurong Zheng, Antoine E Karnoub
Dec 12, 2021·Acta Pharmacologica Sinica·Lin RuJun-Qi Niu