GLP-1 Treatment Improves Diabetic Retinopathy by Alleviating Autophagy through GLP-1R-ERK1/2-HDAC6 Signaling Pathway

International Journal of Medical Sciences
Xiangsheng CaiHongxiang Hui

Abstract

Objective: Apoptosis and autophagy of retinal cells, which may be induced by oxidative stress, are tightly associated with the pathogenesis of diabetic retinopathy (DR). The autophagy induced by oxidative stress is considered as excessively stimulated autophagy, which accelerates the progression of DR. This study aims to investigate the protective effect of GLP-1 treatment on alleviating apoptosis and autophagy of retinal cells in type 2 diabetic rats and reveals its possible mechanism. Methods: Type 2 diabetic rats were induced by fed with high sugar, high fat diet and followed with streptozotocin injection. GLP-1 was applied to treat the diabetic rats for one week after the onset of diabetes. The expressions of oxidative stress-related enzymes, retinal GLP-1R, mitochondria-dependent apoptosis- related genes, autophagy markers, and autophagy-associated pathway genes were studied by Western blotting or immunohistochemistry analysis. Results: GLP-1treatment reduced the levels of NOX3 and SOD2 in DR. The expression of BCL2 was increased, while the levels of caspase3 and LC3B were reduced through GLP-1 treatment in DR. GLP-1 treatment restored the GLP-1R expression and decreased the levels of phosphorylated AKT and phosphorylated ...Continue Reading

Citations

Apr 3, 2019·Brain Injury : [BI]·Damla ErbilHale Yapıcı Eser
Jul 15, 2020·Antioxidants·Hossameldin AbouhishManuela Bartoli
Aug 4, 2019·Journal of Clinical Medicine·Davide CerviaGiovanni Casini
Dec 4, 2019·Frontiers in Neuroscience·Maria Grazia RossinoGiovanni Casini
Apr 1, 2021·Molecular Neurobiology·María F González FleitasRuth E Rosenstein
Apr 13, 2021·Frontiers in Medicine·Qiaoyun GongXun Xu
Jul 9, 2021·Frontiers in Pharmacology·Annagrazia AdornettoRossella Russo
Apr 23, 2019·European Journal of Pharmacology·Jing-Jie PengXiao-Bo Xia
Dec 18, 2021·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Thomas Arendt NielsenChristina Brock

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light microscopy

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