GLP-2, EGF, and the Intestinal Epithelial IGF-1 Receptor Interactions in the Regulation of Crypt Cell Proliferation.

Endocrinology
Zivit FeslerPatricia L Brubaker

Abstract

Glucagon-like peptide-2 (GLP-2) is an intestinotrophic hormone that promotes intestinal growth and proliferation through downstream mediators, including epidermal growth factor (EGF) and insulin-like growth factor-1 (IGF-1). EGF synergistically enhances the proliferative actions of IGF-1 in intestinal cell lines, and both of these factors are known to be essential for the trophic effects of GLP-2 in vivo. However, whether EGF and IGF-1 interact to mediate the proliferative actions of GLP-2 in vivo remains unknown. Normal and knockout (KO) mice lacking the intestinal epithelial IGF-1 receptor (IE-IGF-1R) were therefore treated chronically with EGF and/or long-acting human hGly2GLP-2, followed by determination of intestinal growth parameters. Intestines from control and IE-IGF-1R KO mice were also used to generate organoids (which lack the GLP-2 receptor) and were treated with EGF and/or IGF-1. Combination treatment with EGF and hGly2GLP-2 increased small intestinal weight and crypt-villus height in C57Bl/6 mice in an additive manner, whereas only hGly2GLP-2 treatment increased crypt cell proliferation. However, although combination treatment also increased small intestinal weight and crypt-villus height in IE-IGF-1R KO mice, the...Continue Reading

References

Jan 1, 1994·The Journal of Cell Biology·J C Reed
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·D J DruckerP L Brubaker
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·D G MunroeA Crivici
Aug 13, 1999·The American Journal of Physiology·D W PowellA B West
Mar 11, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·P A KitchenA Forbes
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·M Bjerknes, H Cheng
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 5, 2003·Cellular and Molecular Life Sciences : CMLS·R W C Wong
Jul 30, 2004·Genesis : the Journal of Genetics and Development·Fatima el MarjouSylvie Robine
Jun 18, 2005·JPEN. Journal of Parenteral and Enteral Nutrition·Paul A KitchenJulian R F Walters
Mar 31, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·David L SigaletKeith A Sharkey
Oct 31, 2008·PLoS Biology·Laurent KappelerMartin Holzenberger
Jun 16, 2009·Gastroenterology·Bernardo YustaDaniel J Drucker
Aug 12, 2014·Current Protocols in Mouse Biology·Maxime M MahéNoah F Shroyer
Dec 9, 2014·Current Opinion in Endocrinology, Diabetes, and Obesity·Palle B Jeppesen
Apr 4, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Laurianne Van LandeghemP Kay Lund
Aug 20, 2015·Nature·Dominic GrünAlexander van Oudenaarden
Jan 21, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·David W LimPaul W Wales
May 4, 2017·Cellular and Molecular Gastroenterology and Hepatology·Yongjia FengPeter J Dempsey
Jul 14, 2017·Molecular Metabolism·Pernille WismannJacob Jelsing
Apr 11, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kaori AustinPatricia L Brubaker
Jul 7, 2018·Comprehensive Physiology·Patricia L Brubaker

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiovascular Biology of GLP-1

Glucagon-like peptide 1 (GLP-1) plays a role in glucose metabolism, energy homeostasis, and inflammation suppression. GLP-1 receptor signaling has been shown to impact cardiovascular function. This feed focuses on the role of GLP-1 and GLP-1 receptor agonists on cardiovascular biology.