PMID: 2116897Jun 5, 1990Paper

Glu-69 of the D2 protein in photosystem II is a potential ligand to Mn involved in photosynthetic oxygen evolution

Biochemistry
W F VermaasG Z Shen

Abstract

To probe the involvement of amino acid residues of the D2 protein in the water-splitting process in photosystem II, site-directed mutagenesis was applied to identify D2 residues that might contribute to binding the Mn cluster involved in oxygen evolution. Mutation of Glu-69 to Gln or Val in D2 of the cyanobacterium Synechocystis sp. PCC 6803 was found to lead to a loss of photoautotrophic growth. However, in cells of the Gln mutant (E69Q) a significant Hill reaction rate could be observed upon the start of illumination, but the oxygen evolution rate declined with a half-time of approximately 1 min. Addition of 1 mM Mn2+ stabilized oxygen evolution in E69Q thylakoids. Other divalent cations were ineffective in specific stabilization. When the water-splitting system was bypassed, the rate of electron transport remained stable during illumination, indicating that the inactivation of oxygen evolution is localized in the water-splitting complex. We interpret these observations to indicate that Glu-69 is a Mn ligand and that the loss of oxygen evolution in the E69Q mutant upon turnover of PS II is initiated by changes in the Mn cluster, possibly leading to Mn release from the water-splitting complex. The addition of exogenous Mn to E...Continue Reading

References

Jun 1, 1989·Trends in Biochemical Sciences·A W Rutherford
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R J DebusL McIntosh
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B A Barry, G T Babcock
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W F VermassO Hansson

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Citations

Jan 1, 1993·Photosynthesis Research·W F VermaasB Andersson
Dec 15, 2000·Biochimica Et Biophysica Acta·J H NugentM C Evans
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G T Babcock
May 1, 1996·European Journal of Biochemistry·J H Nugent
Oct 1, 1992·Current Opinion in Genetics & Development·J D Rochaix
Oct 16, 1992·Biochimica Et Biophysica Acta·R J Debus
Sep 26, 2003·Biophysical Journal·Kelly M Halverson, Bridgette A Barry
May 20, 2020·The ISME Journal·Christen L GrettenbergerAnne D Jungblut

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