PMID: 6991342Jul 1, 1980Paper

Glucagon precursors identified by immunoprecipitation of products of cell-free translation of messenger RNA

Diabetes
P K LundJ F Habener

Abstract

Polyadenylated RNA extracted from anglerfish islets was translated in a wheat germ cell-free system containing [35S]methionine in the presence and absence of microsomal membranes prepared from a canine pancreas. Labeled translation products were analyzed by immunoprecipitation with an antiserum to porcine glucagon, followed by electrophoresis of the translation products and immunoprecipitated proteins on SDS polyacrylamide gels. In the absence of microsomal membranes two proteins of Mr = 14,500 and Mr = 12,500 were specifically immunoprecipitated with antiglucagon serum. Addition of microsomal membranes to the translation reactions resulted in a diminution of the labeled protein of Mr = 14,500 and a marked increase in the immunoreactive protein of Mr = 12,500. The protein of Mr = 12,500 was resistant to degradation by proteolytic enzymes added to translation reactions, indicating that it was segregated within microsomal vesicles. These results are consistent with synthesis of anglerfish islet glucagon in the form of a pre-prohormonal precursor (Mr = 14,500) containing a leader sequence that is cotranslationally cleaved from the protein by enzymes associated with microsomal membranes to produce a smaller intermediate prohormonal...Continue Reading

Citations

Jun 18, 1981·The New England Journal of Medicine·R H Unger, L Orci
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P K LundJ F Habener
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L C LopezG F Saunders
Dec 1, 1981·Gut·G B Irvine, R F Murphy
May 17, 2002·Journal of Pediatric Endocrinology & Metabolism : JPEM·Louise RobertsPaul A Fournier
May 14, 2019·Frontiers in Endocrinology·Jens Juul Holst
Jun 1, 1981·Biochemical and Biophysical Research Communications·P K LundJ F Habener

❮ Previous
Next ❯

Related Concepts

Related Feeds

American Diabetes Association Journals

Discover the latest diabetes research published by the journals from the American Diabetes Association.